נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Use of monoclonal antibodies in the purification of an inhibitor of virus replication by affinity chromatography.
Year:
1989
Source of publication :
Journal of General Virology
Authors :
גרה, עבדאללה
;
.
סלומון, רפי
;
.
צדקה, אבי
;
.
שפיגל, שרה
;
.
Volume :
70
Co-Authors:
Gera, A., Virus Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
Sadka, A., Virus Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
Spiegel, S., Virus Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
Salomon, R., Virus Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
Smorodinsky, N.I., Virus Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Mouse monoclonal antibodies (MAbs) were prepared to an inhibitor of virus replication (IVR), released from protoplasts or leaf tissue of hypersensitive tobacco plants infected with tobacco mosaic virus. The MAbs were highly specific for IVR and reduced its antiviral activity. Using these MAbs in affinity chromatography enabled the recovery of purified IVR. SDS-PAGE of the immunoaffinity-purified IVR gave a single Mr 23K band. Immunoblots of IVR from extracts of protoplast or leaf tissue also revealed a single Mr 23K band which suggests that protoplast and tissue IVR are closely related.
Note:
Related Files :
Microbiology
plant
Protoplasts
Tobacco mosaic virus
virus inhibition
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21143
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:41
You may also be interested in
Scientific Publication
Use of monoclonal antibodies in the purification of an inhibitor of virus replication by affinity chromatography.
70
Gera, A., Virus Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
Sadka, A., Virus Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
Spiegel, S., Virus Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
Salomon, R., Virus Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
Smorodinsky, N.I., Virus Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
Use of monoclonal antibodies in the purification of an inhibitor of virus replication by affinity chromatography.
Mouse monoclonal antibodies (MAbs) were prepared to an inhibitor of virus replication (IVR), released from protoplasts or leaf tissue of hypersensitive tobacco plants infected with tobacco mosaic virus. The MAbs were highly specific for IVR and reduced its antiviral activity. Using these MAbs in affinity chromatography enabled the recovery of purified IVR. SDS-PAGE of the immunoaffinity-purified IVR gave a single Mr 23K band. Immunoblots of IVR from extracts of protoplast or leaf tissue also revealed a single Mr 23K band which suggests that protoplast and tissue IVR are closely related.
Scientific Publication
You may also be interested in