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פותח על ידי קלירמאש פתרונות בע"מ -
Production of antiviral and antitumor proteins MAP30 and GAP31 in cucurbits using the plant virus vector ZYMV-AGII
Year:
2002
Authors :
ארזי, צחי
;
.
גל-און, עמית
;
.
Volume :
292
Co-Authors:
Arazi, T., Biochemistry Department, New York University Sch. of Medicine, New York, NY 10016, United States
Huang, P.L., Biochemistry Department, New York University Sch. of Medicine, New York, NY 10016, United States
Zhang, L., Biochemistry Department, New York University Sch. of Medicine, New York, NY 10016, United States
Shiboleth, Y.M., Biochemistry Department, New York University Sch. of Medicine, New York, NY 10016, United States
Gal-On, A., Biochemistry Department, New York University Sch. of Medicine, New York, NY 10016, United States
Lee-Huang, S., Biochemistry Department, New York University Sch. of Medicine, New York, NY 10016, United States
Arazi, T., ViroGene Ltd., Hod Hasharon 45241, Israel
Moshe Shiboleth, Y., ViroGene Ltd., Hod Hasharon 45241, Israel
Lee Huang, P., American Biosciences Inc., Boston, MA 02114, United States
Huang, P.L., American Biosciences Inc., Boston, MA 02114, United States
Zhang, L., Biochemistry Department, New York University School of Medicine, New York, New York, 10016, United States
Lee-Huang, S., Biochemistry Department, New York University School of Medicine, New York, New York, 10016, United States
Gal-On, A., Department of Virology, Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
441
To page:
448
(
Total pages:
8
)
Abstract:
ZYMV-AGII (zucchini yellow mosaic virus-AGII) is a recombinant nonpathogenic potyvirus-based vector system for the expression of foreign genes in cucurbit plants and their edible fruits, including squash, cucumber, melon, watermelon, and pumpkin. MAP30 (Momordica anti-HIV protein, 30 kDa) and GAP31 (Gelonium anti-HIV protein 31 kDa) are multifunctional plant proteins with activity against HIV-1 virus. These proteins are also effective against other viruses, tumor cells, and microbes. We report here the production and characterization of biologically active MAP30 and GAP31 in squash plant by expression of their genes using the ZYMV-AGII vector. Recombinant expressed MAP30 and GAP31 exhibit comparable antiviral, anti-tumor, and antimicrobial activities as their counter- parts from their original plant sources, with EC50s in the ranges of 0.2-0.3 nM for HIV-1. These results demonstrate for the first time the amplification and production of therapeutic proteins, MAP30 and GAP31, in common vegetables. This provides valuable alternative food sources of these antiviral, antitumor, and antimicrobial agents for therapeutic applications. © 2002 Elsevier Science (USA).
Note:
Related Files :
Cucumis sativus
Cucurbita
Cucurbits
gene amplification
gene expression
Zucchini yellow mosaic virus
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/bbrc.2002.6653
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21182
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:42
Scientific Publication
Production of antiviral and antitumor proteins MAP30 and GAP31 in cucurbits using the plant virus vector ZYMV-AGII
292
Arazi, T., Biochemistry Department, New York University Sch. of Medicine, New York, NY 10016, United States
Huang, P.L., Biochemistry Department, New York University Sch. of Medicine, New York, NY 10016, United States
Zhang, L., Biochemistry Department, New York University Sch. of Medicine, New York, NY 10016, United States
Shiboleth, Y.M., Biochemistry Department, New York University Sch. of Medicine, New York, NY 10016, United States
Gal-On, A., Biochemistry Department, New York University Sch. of Medicine, New York, NY 10016, United States
Lee-Huang, S., Biochemistry Department, New York University Sch. of Medicine, New York, NY 10016, United States
Arazi, T., ViroGene Ltd., Hod Hasharon 45241, Israel
Moshe Shiboleth, Y., ViroGene Ltd., Hod Hasharon 45241, Israel
Lee Huang, P., American Biosciences Inc., Boston, MA 02114, United States
Huang, P.L., American Biosciences Inc., Boston, MA 02114, United States
Zhang, L., Biochemistry Department, New York University School of Medicine, New York, New York, 10016, United States
Lee-Huang, S., Biochemistry Department, New York University School of Medicine, New York, New York, 10016, United States
Gal-On, A., Department of Virology, Volcani Center, Bet Dagan, 50250, Israel
Production of antiviral and antitumor proteins MAP30 and GAP31 in cucurbits using the plant virus vector ZYMV-AGII
ZYMV-AGII (zucchini yellow mosaic virus-AGII) is a recombinant nonpathogenic potyvirus-based vector system for the expression of foreign genes in cucurbit plants and their edible fruits, including squash, cucumber, melon, watermelon, and pumpkin. MAP30 (Momordica anti-HIV protein, 30 kDa) and GAP31 (Gelonium anti-HIV protein 31 kDa) are multifunctional plant proteins with activity against HIV-1 virus. These proteins are also effective against other viruses, tumor cells, and microbes. We report here the production and characterization of biologically active MAP30 and GAP31 in squash plant by expression of their genes using the ZYMV-AGII vector. Recombinant expressed MAP30 and GAP31 exhibit comparable antiviral, anti-tumor, and antimicrobial activities as their counter- parts from their original plant sources, with EC50s in the ranges of 0.2-0.3 nM for HIV-1. These results demonstrate for the first time the amplification and production of therapeutic proteins, MAP30 and GAP31, in common vegetables. This provides valuable alternative food sources of these antiviral, antitumor, and antimicrobial agents for therapeutic applications. © 2002 Elsevier Science (USA).
Scientific Publication
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