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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Detection of phytoplasma by polymerase chain reaction of insect feeding medium and its use in determining vectoring ability
Year:
2001
Source of publication :
Phytopathology
Authors :
דוידוביץ', מיכאל
;
.
קליין, מאיר
;
.
Volume :
91
Co-Authors:
Tanne, E., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Boudon-Padieu, E., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Clair, D., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Davidovich, M., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Melamed, S., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Klein, M., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
741
To page:
746
(
Total pages:
6
)
Abstract:
A polymerase chain reaction (PCR)-based method was developed for the detection of phytoplasma in insect feeding medium (sucrose). A correlation was established between the transmissibility of Flavescence dorée phytoplasma in the experimental leafhopper vector Euscelidius variegatus and its detection by PCR in the insect feeding medium. However, phytoplasma were detected in the insects' bodies 3 weeks before they began to transmit. Hence, PCR assays of the sucrose medium reflected phytoplasma vectoring ability probably by detecting it in the insect saliva, whereas detection of phytoplasma in the insect's body did not identify it as a vector. The assay was applied to two field-collected leafhoppers suspected of being phytoplasma vectors in- Israel (Orosius albicinctus and Anaceratagallia laevis). The presence of phytoplasma in the body of specimens of the latter species was assayed by PCR in 1999. Phytoplasmas were detected in insects' bodies throughout the year, with no specific seasonal pattern. In the saliva, however, no phytoplasma could be detected in the autumn. This seasonal pattern supported the validity of the feeding-medium tests and their correlation to the insect's ability to transmit phytoplasma. Transmission assays indicated, to our knowledge for the first time, that O. albicinctus and A. laevis are vectors of phytoplasma in Israel. A simple PCR-based assay is thus provided, circumventing the need for tedious biological assays and enabling epidemiological studies of phytoplasma transmissibility on a large scale.
Note:
Related Files :
Anaceratagallia laevis
Israel
Phytoplasma
Plant Disease
Polymerase Chain Reaction
season
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21187
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:42
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Scientific Publication
Detection of phytoplasma by polymerase chain reaction of insect feeding medium and its use in determining vectoring ability
91
Tanne, E., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Boudon-Padieu, E., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Clair, D., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Davidovich, M., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Melamed, S., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Klein, M., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Detection of phytoplasma by polymerase chain reaction of insect feeding medium and its use in determining vectoring ability
A polymerase chain reaction (PCR)-based method was developed for the detection of phytoplasma in insect feeding medium (sucrose). A correlation was established between the transmissibility of Flavescence dorée phytoplasma in the experimental leafhopper vector Euscelidius variegatus and its detection by PCR in the insect feeding medium. However, phytoplasma were detected in the insects' bodies 3 weeks before they began to transmit. Hence, PCR assays of the sucrose medium reflected phytoplasma vectoring ability probably by detecting it in the insect saliva, whereas detection of phytoplasma in the insect's body did not identify it as a vector. The assay was applied to two field-collected leafhoppers suspected of being phytoplasma vectors in- Israel (Orosius albicinctus and Anaceratagallia laevis). The presence of phytoplasma in the body of specimens of the latter species was assayed by PCR in 1999. Phytoplasmas were detected in insects' bodies throughout the year, with no specific seasonal pattern. In the saliva, however, no phytoplasma could be detected in the autumn. This seasonal pattern supported the validity of the feeding-medium tests and their correlation to the insect's ability to transmit phytoplasma. Transmission assays indicated, to our knowledge for the first time, that O. albicinctus and A. laevis are vectors of phytoplasma in Israel. A simple PCR-based assay is thus provided, circumventing the need for tedious biological assays and enabling epidemiological studies of phytoplasma transmissibility on a large scale.
Scientific Publication
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