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Journal of Virological Methods
Rosner, A., Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, Volcani Center, Bet Dagan 50250, Israel
A method for the differentiation of virus strains based on the shift in electrophoretic mobility of partially annealed RNA transcripts is described. Oppositely oriented RNA transcripts of the NTN- and N-strains of PVY, complementary at their 3′-end variable (strain-specific) region, were annealed to form a partial duplex which moved more slowly in gel than heterologous (NTN + N) unpaired transcripts. Thus, the two virus strains could be identified by annealing to a known reference transcript. The rate of duplex migration was correlated with transcript lengths and could be tightly controlled thereby. Thus, a higher degree of resolution was obtained than with transcript conformation polymorphism, which is empirical and unpredictable in nature. © 2001 Elsevier Science B.V. All rights reserved.
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הספר "אוצר וולקני"
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תנאי שימוש
Differentiating PVYNTN from PVYN by annealing to reference RNA transcripts
97
Rosner, A., Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Differentiating PVYNTN from PVYN by annealing to reference RNA transcripts
A method for the differentiation of virus strains based on the shift in electrophoretic mobility of partially annealed RNA transcripts is described. Oppositely oriented RNA transcripts of the NTN- and N-strains of PVY, complementary at their 3′-end variable (strain-specific) region, were annealed to form a partial duplex which moved more slowly in gel than heterologous (NTN + N) unpaired transcripts. Thus, the two virus strains could be identified by annealing to a known reference transcript. The rate of duplex migration was correlated with transcript lengths and could be tightly controlled thereby. Thus, a higher degree of resolution was obtained than with transcript conformation polymorphism, which is empirical and unpredictable in nature. © 2001 Elsevier Science B.V. All rights reserved.
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