Co-Authors:
Leshem, B., Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan 50 250, Israel
Shaley, D.P., Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan 50 250, Israel
Izhar, S., Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan 50 250, Israel
Abstract:
Development of vitrification has been followed in melon shoot tips cultured on solid and liquid media which contained growth factors in various concentrations. Cytokinin was found to be the primary inducer, without which vitrification did not occur in spite of the presence in the culture of other accepted inducers. Spontaneous vitrification appeared on non-inductive media but in a low percentage. Vitrification differed in appearance and frequency, depending on the kind of medium. On solid medium vitrification gradually increased with time, whereas on liquid medium it was an 'all-or-nothing' effect. On solid medium, all the vitrified plantlets turned into callus after two or three subcultures. It is suggested that vitrification is the bud's reaction to an excess of cytokinin. © 1988 Annals of Botany Company.
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