נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Length polymorphism and homologies of microsatellites in several Cucurbitaceae species
Year:
1996
Source of publication :
Theoretical and Applied Genetics
Authors :
דנין-פולג, יעל
;
.
לביא, אורי
;
.
צורי, גליל
;
.
קציר, נורית
;
.
קרחי, צבי
;
.
Volume :
93
Co-Authors:

Katzir, N., Department of Vegetable Crops, Agricultural Research Organization, Northern Research Center, Newe Ya'an, P.O.B. 90000, Haifa 31900, Israel
Danin-Poleg, Y., Department of Vegetable Crops, Agricultural Research Organization, Northern Research Center, Newe Ya'an, P.O.B. 90000, Haifa 31900, Israel
Tzuri, G., Department of Vegetable Crops, Agricultural Research Organization, Northern Research Center, Newe Ya'an, P.O.B. 90000, Haifa 31900, Israel
Karchi, Z., Department of Vegetable Crops, Agricultural Research Organization, Northern Research Center, Newe Ya'an, P.O.B. 90000, Haifa 31900, Israel
Lavi, U., Institute of Horticulture, Agricultural Research Organization, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Cregan, P.B., USDA-ARS, BARC-West, Beltsville, MD 20705, United States

Facilitators :
From page:
1282
To page:
1290
(
Total pages:
9
)
Abstract:
The objectives of this research were to assess (1) the degree of Simple Sequence Repeats (SSR) DNA length polymorphism in melon (Cucumis melo L.) and other species within the Cucurbitaceae family and (2) the possibility of utilizing SSRs flanking primers from single species to other genera or species of Cucurbitaceae. Five melon (CT/GA)n SSRs were isolated from a genomic library. Two cucumber (Cucumis sativus L.) SSRs were detected through a search of DNA sequence databases, one contained a (CT)8 repeat, the other a (AT)13 repeat. The seven SSRs were used to test a diverse sample of Cucurbitaceae, including 8 melon, 11 cucumber, 5 squash, 1 pumpkin, and 3 watermelon genotypes. Five of the seven SSRs detected length polymorphism among the 8 melon genotypes. PCR amplification revealed between three and five length variants (alleles) for each SSR locus, with gene diversity values ranging from 0.53 to 0.75. Codominant segregation of the alleles among F2 progeny was demonstrated for each of the five SSR loci. Four of the seven SSRs detected polymorphism among the 11 cucumber genotypes, with gene diversity values ranging between 0.18 and 0.64. Primers specific to SSRs of C. melo and C. sativus also amplified DNA extracted from genotypes belonging to other genera of the Cucurbitaceae family.
Note:
Related Files :
Cucumis melo L.
Cucurbitaceae
DNA markers
Melon
Microsatellites
Simple Sequence Repeats (SSR)
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21283
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:42
Scientific Publication
Length polymorphism and homologies of microsatellites in several Cucurbitaceae species
93

Katzir, N., Department of Vegetable Crops, Agricultural Research Organization, Northern Research Center, Newe Ya'an, P.O.B. 90000, Haifa 31900, Israel
Danin-Poleg, Y., Department of Vegetable Crops, Agricultural Research Organization, Northern Research Center, Newe Ya'an, P.O.B. 90000, Haifa 31900, Israel
Tzuri, G., Department of Vegetable Crops, Agricultural Research Organization, Northern Research Center, Newe Ya'an, P.O.B. 90000, Haifa 31900, Israel
Karchi, Z., Department of Vegetable Crops, Agricultural Research Organization, Northern Research Center, Newe Ya'an, P.O.B. 90000, Haifa 31900, Israel
Lavi, U., Institute of Horticulture, Agricultural Research Organization, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Cregan, P.B., USDA-ARS, BARC-West, Beltsville, MD 20705, United States

Length polymorphism and homologies of microsatellites in several Cucurbitaceae species
The objectives of this research were to assess (1) the degree of Simple Sequence Repeats (SSR) DNA length polymorphism in melon (Cucumis melo L.) and other species within the Cucurbitaceae family and (2) the possibility of utilizing SSRs flanking primers from single species to other genera or species of Cucurbitaceae. Five melon (CT/GA)n SSRs were isolated from a genomic library. Two cucumber (Cucumis sativus L.) SSRs were detected through a search of DNA sequence databases, one contained a (CT)8 repeat, the other a (AT)13 repeat. The seven SSRs were used to test a diverse sample of Cucurbitaceae, including 8 melon, 11 cucumber, 5 squash, 1 pumpkin, and 3 watermelon genotypes. Five of the seven SSRs detected length polymorphism among the 8 melon genotypes. PCR amplification revealed between three and five length variants (alleles) for each SSR locus, with gene diversity values ranging from 0.53 to 0.75. Codominant segregation of the alleles among F2 progeny was demonstrated for each of the five SSR loci. Four of the seven SSRs detected polymorphism among the 11 cucumber genotypes, with gene diversity values ranging between 0.18 and 0.64. Primers specific to SSRs of C. melo and C. sativus also amplified DNA extracted from genotypes belonging to other genera of the Cucurbitaceae family.
Scientific Publication
You may also be interested in