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פותח על ידי קלירמאש פתרונות בע"מ -
Effect of temperature during the incubation period on tibial growth plate chondrocyte differentiation and the incidence of tibial dyschondroplasia
Year:
2007
Source of publication :
Poultry Science
Authors :
גנין, אולגה
;
.
פינס, מרק
;
.
Volume :
86
Co-Authors:
Yalçin, S., Department of Animal Science, Faculty of Agriculture, Ege University, Izmir, Turkey
Molayoǧlu, H.B., Department of Animal Science, Faculty of Agriculture, Ege University, Izmir, Turkey
Baka, M., Department of Embryology and Histology, Faculty of Medicine, Ege University, Izmir, Turkey
Genin, O., Institute of Animal Sciences, Volcani Center, Bet Dagan, Israel
Pines, M., Institute of Animal Sciences, Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
1772
To page:
1783
(
Total pages:
12
)
Abstract:
Tibial dyschondroplasia (TD) is one of the most prevalent skeletal abnormalities in avian species, causing enormous economic losses and major animal welfare problems. Irregular cell differentiation of the chondrocytes that populate the growth plate has been hypothesized to be involved in the etiology of the disease. We evaluated the effect of incubation temperature at various stages of embryo development and bone formation on growth plate chondrocyte differentiation and the incidence of TD. Eggs were incubated either at a control temperature of 37.8°C, or at 36.9 or 39°C, each for 6 h/d, during early (0 to 8 d) or late (10 to 18 d) embryo development. At 14 d of incubation and at hatch, tibias were collected and weighed, and their ash and calcium contents were determined. Growth plate chondrocyte differentiation was evaluated by alkaline phosphatase activity and collagen type II and osteopontin gene expression. In addition, the level of the heat-shock protein 90 (Hsp90) was evaluated by immunohistochemistry. The rest of the chicks were raised to 49 d and the incidence of TD was recorded. The incidence of TD increased only when the temperature was altered at the early stages of embryo development, and it was correlated with an increase in tibia ash but not with tibia weight or calcium content. Moreover, increased TD incidence was correlated with delayed chondrocyte differentiation. Early changes in incubation temperature caused an increase in the level of Hsp90 in articular and differentiated chondrocytes of the hypertrophic zone and in the numbers of distinct undifferentiated chondrocytes arranged in columns in the proliferative zone of the growth plate. In summary, the early stages of embryo development and bone formation are of utmost importantance for appropriate growth plate chondrocyte differentiation, and any temperature deviation will increase the subsequent incidence of TD. The increase in TD incidence is probably the result of delayed Hsp90-driven chondrocyte differentiation, supporting the hypothesis that TD is the result of abnormal chondrocyte differentiation. ©2007 Poultry Science Association Inc.
Note:
Related Files :
Animal
Animals
Female
Growth plate
Male
metabolism
mineral
Osteochondrodysplasias
Pathology
temperature
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21341
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:43
Scientific Publication
Effect of temperature during the incubation period on tibial growth plate chondrocyte differentiation and the incidence of tibial dyschondroplasia
86
Yalçin, S., Department of Animal Science, Faculty of Agriculture, Ege University, Izmir, Turkey
Molayoǧlu, H.B., Department of Animal Science, Faculty of Agriculture, Ege University, Izmir, Turkey
Baka, M., Department of Embryology and Histology, Faculty of Medicine, Ege University, Izmir, Turkey
Genin, O., Institute of Animal Sciences, Volcani Center, Bet Dagan, Israel
Pines, M., Institute of Animal Sciences, Volcani Center, Bet Dagan, Israel
Effect of temperature during the incubation period on tibial growth plate chondrocyte differentiation and the incidence of tibial dyschondroplasia
Tibial dyschondroplasia (TD) is one of the most prevalent skeletal abnormalities in avian species, causing enormous economic losses and major animal welfare problems. Irregular cell differentiation of the chondrocytes that populate the growth plate has been hypothesized to be involved in the etiology of the disease. We evaluated the effect of incubation temperature at various stages of embryo development and bone formation on growth plate chondrocyte differentiation and the incidence of TD. Eggs were incubated either at a control temperature of 37.8°C, or at 36.9 or 39°C, each for 6 h/d, during early (0 to 8 d) or late (10 to 18 d) embryo development. At 14 d of incubation and at hatch, tibias were collected and weighed, and their ash and calcium contents were determined. Growth plate chondrocyte differentiation was evaluated by alkaline phosphatase activity and collagen type II and osteopontin gene expression. In addition, the level of the heat-shock protein 90 (Hsp90) was evaluated by immunohistochemistry. The rest of the chicks were raised to 49 d and the incidence of TD was recorded. The incidence of TD increased only when the temperature was altered at the early stages of embryo development, and it was correlated with an increase in tibia ash but not with tibia weight or calcium content. Moreover, increased TD incidence was correlated with delayed chondrocyte differentiation. Early changes in incubation temperature caused an increase in the level of Hsp90 in articular and differentiated chondrocytes of the hypertrophic zone and in the numbers of distinct undifferentiated chondrocytes arranged in columns in the proliferative zone of the growth plate. In summary, the early stages of embryo development and bone formation are of utmost importantance for appropriate growth plate chondrocyte differentiation, and any temperature deviation will increase the subsequent incidence of TD. The increase in TD incidence is probably the result of delayed Hsp90-driven chondrocyte differentiation, supporting the hypothesis that TD is the result of abnormal chondrocyte differentiation. ©2007 Poultry Science Association Inc.
Scientific Publication
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