אסיף מאגר המחקר החקלאי

Movement of protein and macromolecules between host plants and the parasitic weed Phelipanche aegyptiaca Pers

Year:

2011

Source of publication :

Authors :

Volume :

30

Co-Authors:

Aly, R., Department of Plant Pathology and Weed Research, ARO, The Volcani Center Newe-Yaar Research Center, P.O. Box 1021, 30095 Ramat yeshai, Israel
Hamamouch, N., Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA 24061-0390, United States, Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695, United States
Abu-Nassar, J., Department of Plant Pathology and Weed Research, ARO, The Volcani Center Newe-Yaar Research Center, P.O. Box 1021, 30095 Ramat yeshai, Israel
Wolf, S., The Institute of Plant Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, 76100 Rehovot, Israel
Joel, D.M., Department of Plant Pathology and Weed Research, ARO, The Volcani Center Newe-Yaar Research Center, P.O. Box 1021, 30095 Ramat yeshai, Israel
Eizenberg, H., Department of Plant Pathology and Weed Research, ARO, The Volcani Center Newe-Yaar Research Center, P.O. Box 1021, 30095 Ramat yeshai, Israel
Kaisler, E., The Institute of Plant Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, 76100 Rehovot, Israel
Cramer, C., Arkansas Biosciences Institute, Arkansas State University, State University, Jonesboro, AR 72467, United States
Gal-On, A., Department of Plant Pathology and Weed Research, ARO, The Volcani Center Newe-Yaar Research Center, P.O. Box 1021, 30095 Ramat yeshai, Israel
Westwood, J.H., Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA 24061-0390, United States

Facilitators :

Link :

Abstract:

Little is known about the translocation of proteins and other macromolecules from a host plant to the parasitic weed Phelipanche spp. Long-distance movement of proteins between host and parasite was explored using transgenic tomato plants expressing green fluorescent protein (GFP) in their companion cells. We further used fluorescent probes of differing molecular weights to trace vascular continuity between the host plant and the parasite. Accumulation of GFP was observed in the central vascular bundle of leaves and in the root phloem of transgenic tomato plants expressing GFP under the regulation of AtSUC2 promoter. When transgenic tomato plants expressing GFP were parasitized with P. aegyptiaca, extensive GFP was translocated from the host phloem to the parasite phloem and accumulated in both Phelipanche tubercles and shoots. No movement of GFP to the parasite was observed when tobacco plants expressing GFP targeted to the ER were parasitized with P. aegyptiaca. Experiments using fluorescent probes of differing molecular weights to trace vascular continuity between the host plant and the parasite demonstrated that Phelipanche absorbs dextrans up to 70 kDa in size from the host and that this movement can be bi-directional. In the present study, we prove for the first time delivery of proteins from host to the parasitic weed P. aegyptiaca via phloem connections, providing information for developing parasite resistance strategies. © 2011 Springer-Verlag.

Note:

Related Files :

תוכן קשור

More details

You may also be interested in