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פותח על ידי קלירמאש פתרונות בע"מ -
ORF 5 of Grapevine Virus A Encodes a Nucleic Acid-Binding Protein and Affects Pathogenesis
Year:
2003
Source of publication :
Virus Genes
Authors :
גפני, רון
;
.
מוואסי, מוניר
;
.
Volume :
27
Co-Authors:
Galiakparov, N., Volcani Center, Department of Virology, Tolkovski Laboratory, Bet Dagan 50250, Israel, Hebrew University, Fac. Agric., Food, Environ. Qual. S., Institute of Plant Sciences, Rehovot, 76100, Israel
Tanne, E., Volcani Center, Department of Virology, Tolkovski Laboratory, Bet Dagan 50250, Israel
Mawassi, M., Volcani Center, Department of Virology, Tolkovski Laboratory, Bet Dagan 50250, Israel
Gafny, R., Volcani Center, Department of Virology, Tolkovski Laboratory, Bet Dagan 50250, Israel
Sela, I., Hebrew University, Fac. Agric., Food, Environ. Qual. S., Institute of Plant Sciences, Rehovot, 76100, Israel
Facilitators :
From page:
257
To page:
262
(
Total pages:
6
)
Abstract:
A previous functional analysis of the genome of grapevine virus A (GVA) was not conclusive as to the role of open reading frame 5 (ORF 5). This ORF encodes a 10-kDa protein (p10) carrying two distinct domains: a basic, arginine-rich domain and a zinc-finger domain. P10 was cloned and expressed in Escherichia coli, and was shown by northwestern assays to interact with nucleic acids. In-frame deletion of the basic region abolished P10's nucleic acid-binding capability, whereas substitution of cysteine residues by serine in the zinc-finger domain did not affect binding. These mutations were inserted into the full-length infectious clone. It has been shown that ORF 5 mutations do not affect replication of GVA-RNA. However, plants inoculated with the aforementioned mutations did not develop symptoms, and Western blot analysis revealed markedly reduced expression of the movement protein (the product of ORF 3).
Note:
Related Files :
Base Sequence
Molecular Sequence Data
nucleic acid
RNA viruses
Sequence Homology, Amino Acid
virus genome
Vitis
עוד תגיות
תוכן קשור
More details
DOI :
10.1023/A:1026395815980
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21397
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:43
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Scientific Publication
ORF 5 of Grapevine Virus A Encodes a Nucleic Acid-Binding Protein and Affects Pathogenesis
27
Galiakparov, N., Volcani Center, Department of Virology, Tolkovski Laboratory, Bet Dagan 50250, Israel, Hebrew University, Fac. Agric., Food, Environ. Qual. S., Institute of Plant Sciences, Rehovot, 76100, Israel
Tanne, E., Volcani Center, Department of Virology, Tolkovski Laboratory, Bet Dagan 50250, Israel
Mawassi, M., Volcani Center, Department of Virology, Tolkovski Laboratory, Bet Dagan 50250, Israel
Gafny, R., Volcani Center, Department of Virology, Tolkovski Laboratory, Bet Dagan 50250, Israel
Sela, I., Hebrew University, Fac. Agric., Food, Environ. Qual. S., Institute of Plant Sciences, Rehovot, 76100, Israel
ORF 5 of Grapevine Virus A Encodes a Nucleic Acid-Binding Protein and Affects Pathogenesis
A previous functional analysis of the genome of grapevine virus A (GVA) was not conclusive as to the role of open reading frame 5 (ORF 5). This ORF encodes a 10-kDa protein (p10) carrying two distinct domains: a basic, arginine-rich domain and a zinc-finger domain. P10 was cloned and expressed in Escherichia coli, and was shown by northwestern assays to interact with nucleic acids. In-frame deletion of the basic region abolished P10's nucleic acid-binding capability, whereas substitution of cysteine residues by serine in the zinc-finger domain did not affect binding. These mutations were inserted into the full-length infectious clone. It has been shown that ORF 5 mutations do not affect replication of GVA-RNA. However, plants inoculated with the aforementioned mutations did not develop symptoms, and Western blot analysis revealed markedly reduced expression of the movement protein (the product of ORF 3).
Scientific Publication
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