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Development of a quantitative tool for measuring changes in the coefficient of conductivity of plasmodesmata induced by developmental, biotic, and abiotic signals
Year:
2005
Source of publication :
Protoplasma
Authors :
ליארזי, אורנה
;
.
Volume :
225
Co-Authors:
Liarzi, O., Department of Plant Sciences, George S. Wise Fac. of Life Sciences, Tel Aviv University, Tel Aviv, Israel, Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel
Epel, B.L., Department of Plant Sciences, George S. Wise Fac. of Life Sciences, Tel Aviv University, Tel Aviv, Israel, Department of Plant Sciences, George S. Wise Fac. of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Facilitators :
From page:
67
To page:
76
(
Total pages:
10
)
Abstract:
The regulation of intercellular and interorgan communication is pivotal for cell fate decisions in plant development and probably plays a significant role in the systemic regulation of gene expression and in defense reactions against pathogens or other biotic and abiotic environmental factors. In plants, symplasmic cell-to-cell communication is provided by plasmodesmata (Pd), coaxial membranous tunnels that span cell walls interconnecting adjacent cytoplasms. Macromolecules, proteins, and RNA may be transported through Pd by passive diffusion or by a facilitated mechanism. A quantitative tool was developed to measure the coefficient of conductivity, C(Pd), for diffusion-driven transport via Pd and to assess changes in the coefficient induced by developmental, biotic and abiotic signals. GFPC(Pd), the coefficient of conductivity for cell-to-cell spread of green-fluorescent protein (GFP), a protein with a Stokes radius of 2.82 nm, was determined in epidermal cells of sink and source leaves of wild-type and transgenic Nicotiana benthamiana plants expressing the movement protein of tobacco mosaic virus (MPTMV) incubated both in dark and light and at 16 and 25°C. Under all conditions, Pd in source leaves conducted macromolecules, with GFPC(Pd)sink > GFPC(Pd)source. Light down-regulated GFPC(Pd) (all conditions); down-regulation was stronger for sink cells. The effect of MPTMV on GFPC(Pd) between epidermal cells was dependent on temperature and leaf development; at 16°C, MPTMV down-regulated GFPC(Pd) only in source leaves, while at 25°C, MPTMV had no significant effect. This quantitative tool should be useful for investigating differences in Pd conductivity that are induced by mutations or silencing. © Springer-Verlag 2005.
Note:
Related Files :
Genetics
Growth, Development and Aging
light
light effect
metabolism
temperature
Tobacco mosaic virus
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00709-004-0079-x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21406
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:43
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Scientific Publication
Development of a quantitative tool for measuring changes in the coefficient of conductivity of plasmodesmata induced by developmental, biotic, and abiotic signals
225
Liarzi, O., Department of Plant Sciences, George S. Wise Fac. of Life Sciences, Tel Aviv University, Tel Aviv, Israel, Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel
Epel, B.L., Department of Plant Sciences, George S. Wise Fac. of Life Sciences, Tel Aviv University, Tel Aviv, Israel, Department of Plant Sciences, George S. Wise Fac. of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Development of a quantitative tool for measuring changes in the coefficient of conductivity of plasmodesmata induced by developmental, biotic, and abiotic signals
The regulation of intercellular and interorgan communication is pivotal for cell fate decisions in plant development and probably plays a significant role in the systemic regulation of gene expression and in defense reactions against pathogens or other biotic and abiotic environmental factors. In plants, symplasmic cell-to-cell communication is provided by plasmodesmata (Pd), coaxial membranous tunnels that span cell walls interconnecting adjacent cytoplasms. Macromolecules, proteins, and RNA may be transported through Pd by passive diffusion or by a facilitated mechanism. A quantitative tool was developed to measure the coefficient of conductivity, C(Pd), for diffusion-driven transport via Pd and to assess changes in the coefficient induced by developmental, biotic and abiotic signals. GFPC(Pd), the coefficient of conductivity for cell-to-cell spread of green-fluorescent protein (GFP), a protein with a Stokes radius of 2.82 nm, was determined in epidermal cells of sink and source leaves of wild-type and transgenic Nicotiana benthamiana plants expressing the movement protein of tobacco mosaic virus (MPTMV) incubated both in dark and light and at 16 and 25°C. Under all conditions, Pd in source leaves conducted macromolecules, with GFPC(Pd)sink > GFPC(Pd)source. Light down-regulated GFPC(Pd) (all conditions); down-regulation was stronger for sink cells. The effect of MPTMV on GFPC(Pd) between epidermal cells was dependent on temperature and leaf development; at 16°C, MPTMV down-regulated GFPC(Pd) only in source leaves, while at 25°C, MPTMV had no significant effect. This quantitative tool should be useful for investigating differences in Pd conductivity that are induced by mutations or silencing. © Springer-Verlag 2005.
Scientific Publication
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