נגישות
menu      
חיפוש מתקדם
Journal of Virological Methods
Shargil, D., Institute of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Zemach, H., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Belausov, E., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Lachman, O., Institute of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Kamenetsky, R., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Dombrovsky, A., Institute of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Cucumber green mottle mosaic virus (CGMMV), which belongs to the genus Tobamovirus, is a major pathogen of cucurbit crops grown indoors and in open fields. Currently, immunology (e.g., ELISA) and molecular amplification techniques (e.g., RT-PCR) are employed extensively for virus detection in plant tissues and commercial seed lots diagnostics. In this study, a fluorescent in situ hybridization (FISH) technique, using oligonucleotides whose 5'-terminals were labeled with red cyanine 3 (Cy3) or green fluorescein isothiocyanate (FITC), was developed for the visualization of the pathogen in situ. This simple and reliable method allows detection and localization of CGMMV in the vegetative and reproductive tissues of cucumber and melon. When this technique was applied in male flowers, anther tissues were found to be infected; whereas the pollen grains were found to be virus-free. These results have meaningful epidemiological implications for the management of CGMMV, particularly with regard to virus transfer via seed and the role of insects as CGMMV vectors. © 2015 Elsevier B.V.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Development of a fluorescent in situ hybridization (FISH) technique for visualizing CGMMV in plant tissues
223
Shargil, D., Institute of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Zemach, H., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Belausov, E., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Lachman, O., Institute of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Kamenetsky, R., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Dombrovsky, A., Institute of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Development of a fluorescent in situ hybridization (FISH) technique for visualizing CGMMV in plant tissues
Cucumber green mottle mosaic virus (CGMMV), which belongs to the genus Tobamovirus, is a major pathogen of cucurbit crops grown indoors and in open fields. Currently, immunology (e.g., ELISA) and molecular amplification techniques (e.g., RT-PCR) are employed extensively for virus detection in plant tissues and commercial seed lots diagnostics. In this study, a fluorescent in situ hybridization (FISH) technique, using oligonucleotides whose 5'-terminals were labeled with red cyanine 3 (Cy3) or green fluorescein isothiocyanate (FITC), was developed for the visualization of the pathogen in situ. This simple and reliable method allows detection and localization of CGMMV in the vegetative and reproductive tissues of cucumber and melon. When this technique was applied in male flowers, anther tissues were found to be infected; whereas the pollen grains were found to be virus-free. These results have meaningful epidemiological implications for the management of CGMMV, particularly with regard to virus transfer via seed and the role of insects as CGMMV vectors. © 2015 Elsevier B.V.
Scientific Publication
You may also be interested in