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פותח על ידי קלירמאש פתרונות בע"מ -
Development of a fluorescent in situ hybridization (FISH) technique for visualizing CGMMV in plant tissues
Year:
2015
Source of publication :
Journal of Virological Methods
Authors :
בלאוסוב, אדוארד
;
.
דומברובסקי, אביב
;
.
לכמן, עודד
;
.
צמח, חניתה
;
.
קמנצקי, רינה
;
.
שרגיל, דורית
;
.
Volume :
223
Co-Authors:
Shargil, D., Institute of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Zemach, H., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Belausov, E., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Lachman, O., Institute of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Kamenetsky, R., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Dombrovsky, A., Institute of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Facilitators :
From page:
55
To page:
60
(
Total pages:
6
)
Abstract:
Cucumber green mottle mosaic virus (CGMMV), which belongs to the genus Tobamovirus, is a major pathogen of cucurbit crops grown indoors and in open fields. Currently, immunology (e.g., ELISA) and molecular amplification techniques (e.g., RT-PCR) are employed extensively for virus detection in plant tissues and commercial seed lots diagnostics. In this study, a fluorescent in situ hybridization (FISH) technique, using oligonucleotides whose 5'-terminals were labeled with red cyanine 3 (Cy3) or green fluorescein isothiocyanate (FITC), was developed for the visualization of the pathogen in situ. This simple and reliable method allows detection and localization of CGMMV in the vegetative and reproductive tissues of cucumber and melon. When this technique was applied in male flowers, anther tissues were found to be infected; whereas the pollen grains were found to be virus-free. These results have meaningful epidemiological implications for the management of CGMMV, particularly with regard to virus transfer via seed and the role of insects as CGMMV vectors. © 2015 Elsevier B.V.
Note:
Related Files :
Cucumber mosaic virus
Cucumis sativus
Cucurbitaceae
Plant Disease
Plant Diseases
pollen (external)
Virology
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.jviromet.2015.07.014
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21428
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:44
Scientific Publication
Development of a fluorescent in situ hybridization (FISH) technique for visualizing CGMMV in plant tissues
223
Shargil, D., Institute of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Zemach, H., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Belausov, E., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Lachman, O., Institute of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Kamenetsky, R., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Dombrovsky, A., Institute of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan, Israel
Development of a fluorescent in situ hybridization (FISH) technique for visualizing CGMMV in plant tissues
Cucumber green mottle mosaic virus (CGMMV), which belongs to the genus Tobamovirus, is a major pathogen of cucurbit crops grown indoors and in open fields. Currently, immunology (e.g., ELISA) and molecular amplification techniques (e.g., RT-PCR) are employed extensively for virus detection in plant tissues and commercial seed lots diagnostics. In this study, a fluorescent in situ hybridization (FISH) technique, using oligonucleotides whose 5'-terminals were labeled with red cyanine 3 (Cy3) or green fluorescein isothiocyanate (FITC), was developed for the visualization of the pathogen in situ. This simple and reliable method allows detection and localization of CGMMV in the vegetative and reproductive tissues of cucumber and melon. When this technique was applied in male flowers, anther tissues were found to be infected; whereas the pollen grains were found to be virus-free. These results have meaningful epidemiological implications for the management of CGMMV, particularly with regard to virus transfer via seed and the role of insects as CGMMV vectors. © 2015 Elsevier B.V.
Scientific Publication
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