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פותח על ידי קלירמאש פתרונות בע"מ -
Reduced intracellular content of methotrexate in an isolated MTX-resistant cell line of Nicotiana plumbaginifolia
Year:
1991
Source of publication :
Plant Cell Reports
Authors :
ברג, רבקה
;
.
שבתאי, שרה
;
.
Volume :
10
Co-Authors:
Facilitators :
From page:
269
To page:
272
(
Total pages:
4
)
Abstract:
In plant cells methotrexate (MTX) may exert its toxic effect through several mechanisms, including inhibition of its target protein dihydrofolate reductase. Resistance based on a mechanism operating before MTX binds to proteins should confer protection to plant cells. A methotrexate-resistant cell line of Nicotiana plumbaginifolia was isolated by a stepwise selection procedure. This cell line survived in the presence of 10 μM MTX which is 50-100 fold higher than the lethal dose for the wild type cells. Neither alteration in kinetic characteristics of dihydrofolate reductase, nor elevated binding capacity of 3H-MTX to target protein(s), were observed. However, in comparison with wild type cells, markedly lower amounts of intracellular 3H-MTX were found after the selected cell line was incubated with 3H-MTX, indicating that either reduced uptake or enhanced efflux of MTX is the major reason for MTX-resistance in this cell line. © 1991 Springer-Verlag.
Note:
Related Files :
cell culture
methotrexate-resistance
methotrexate-uptake
Nicotiana plumbaginifolia
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00232573
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21470
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:44
Scientific Publication
Reduced intracellular content of methotrexate in an isolated MTX-resistant cell line of Nicotiana plumbaginifolia
10
Reduced intracellular content of methotrexate in an isolated MTX-resistant cell line of Nicotiana plumbaginifolia
In plant cells methotrexate (MTX) may exert its toxic effect through several mechanisms, including inhibition of its target protein dihydrofolate reductase. Resistance based on a mechanism operating before MTX binds to proteins should confer protection to plant cells. A methotrexate-resistant cell line of Nicotiana plumbaginifolia was isolated by a stepwise selection procedure. This cell line survived in the presence of 10 μM MTX which is 50-100 fold higher than the lethal dose for the wild type cells. Neither alteration in kinetic characteristics of dihydrofolate reductase, nor elevated binding capacity of 3H-MTX to target protein(s), were observed. However, in comparison with wild type cells, markedly lower amounts of intracellular 3H-MTX were found after the selected cell line was incubated with 3H-MTX, indicating that either reduced uptake or enhanced efflux of MTX is the major reason for MTX-resistance in this cell line. © 1991 Springer-Verlag.
Scientific Publication
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