Co-Authors:
Wang, Z., Department of Fruit Tree Sciences, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China
Zhao, F., Key Laboratory of Functional Dairy Science of Chinese Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
Zhao, X., Department of Fruit Tree Sciences, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China
Ge, H., Department of Fruit Tree Sciences, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China
Chai, L., Department of Fruit Tree Sciences, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China
Chen, S., Key Laboratory of Functional Dairy Science of Chinese Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
Perl, A., Department of Fruit Tree Sciences, Agricultural Research Organization, The Volcani Center, Israel
Ma, H., Department of Fruit Tree Sciences, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China
Abstract:
Gibberellin (GA) is widely used in the table grape and raisin industries to enlarge the berries of seedless varieties. However, the mechanism underlying its berry-sizing effect is poorly understood. In this study, clusters of Centennial Seedless (Vitis vinifera L.) were treated with 30ppm GA 3 on day 12 after flowering, and berries were sampled at development stages I, II and III for proteomic analysis. Among the 1479 proteins detected on 2-DE maps, 19, 70 and 69 spots in stages I, II and III, respectively, showed an at least twofold difference in volume between treatments and controls. Of these, 125 proteins were successfully identified and assigned to eight functional groups, chief among them are metabolism and energy, stress response, expression regulation and cytoskeleton proteins. Stress-response proteins were predominantly down-regulated in GA 3-treated berries in stages I and II, and significantly up-regulated in stage III. Up-regulation of cytoskeleton, cell-wall modification and other important proteins was found in the two latter stages of berry development. Our proteomic results and subsequent validation revealed, for the first time, the role of redox homeostasis in GA 3-induced berry enlargement and markedly remodeled cellular protein expression in treated berries. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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