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Calcified Tissue International
Benayahu, D., Department of Cell Biology, The Weizmann Institute of Science, Rehovot, 76100, Israel
Kompier, R., Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Shamay, A., Division of Orthopedics, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, 66798, Israel
Kadouri, A., Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Zipori, D., Department of Cell Biology, The Weizmann Institute of Science, Rehovot, 76100, Israel
Wientroub, S., Division of Orthopedics, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, 66798, Israel, Department of Pediatric Orthopedics, Dana Children's Hospital, Tel-Aviv Medical Center, Tel-Aviv, 64239, Israel
The present study describes a new three-dimensional (3-D) culture system that enables the maintenance and phenotypic expression of bone marrow stromal osteoblasts. This culture substratum is advantageous in that it provides suitable conditions for attachment, growth, and differentiation of cells forming 3-D layers. The MBA-15 cell line was grown in unlimited quantities on 3-D Fibro-Cel carriers. These cells mineralized when exposed to ascorbic acid and β-glycerophosphate (βGP). Under these mineralization conditions, mRNA expressions of procollagen α2(I) and [3H]-proline-labeled protein were increased. The expression of mRNA for osteonectin and to a lesser extent, for osteopontin was increased, whereas alkaline phosphatase and biglycan remained unaffected under similar conditions. Exposure of mineralizing cultures to dexamethasone reduced mRNA of procollagen α2(I) and osteonectin to control level. Scanning electron microscopy revealed that cells were grown along the fabric's fibers and produced collagen fiberils. Under appropriate conditions, extensive mineralization had taken place. The mineralization process involves the formation of calcospherites, and correlates with an increase in calcium content. The Fibro-Cel carriers enable formation of 3-D architecture and mineralized tissue in vitro. © 1994 Springer-Verlag New York Inc.
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הספר "אוצר וולקני"
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תנאי שימוש
Mineralization of marrow-stromal osteoblasts MBA-15 on three-dimensional carriers
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Benayahu, D., Department of Cell Biology, The Weizmann Institute of Science, Rehovot, 76100, Israel
Kompier, R., Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Shamay, A., Division of Orthopedics, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, 66798, Israel
Kadouri, A., Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Zipori, D., Department of Cell Biology, The Weizmann Institute of Science, Rehovot, 76100, Israel
Wientroub, S., Division of Orthopedics, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, 66798, Israel, Department of Pediatric Orthopedics, Dana Children's Hospital, Tel-Aviv Medical Center, Tel-Aviv, 64239, Israel
Mineralization of marrow-stromal osteoblasts MBA-15 on three-dimensional carriers
The present study describes a new three-dimensional (3-D) culture system that enables the maintenance and phenotypic expression of bone marrow stromal osteoblasts. This culture substratum is advantageous in that it provides suitable conditions for attachment, growth, and differentiation of cells forming 3-D layers. The MBA-15 cell line was grown in unlimited quantities on 3-D Fibro-Cel carriers. These cells mineralized when exposed to ascorbic acid and β-glycerophosphate (βGP). Under these mineralization conditions, mRNA expressions of procollagen α2(I) and [3H]-proline-labeled protein were increased. The expression of mRNA for osteonectin and to a lesser extent, for osteopontin was increased, whereas alkaline phosphatase and biglycan remained unaffected under similar conditions. Exposure of mineralizing cultures to dexamethasone reduced mRNA of procollagen α2(I) and osteonectin to control level. Scanning electron microscopy revealed that cells were grown along the fabric's fibers and produced collagen fiberils. Under appropriate conditions, extensive mineralization had taken place. The mineralization process involves the formation of calcospherites, and correlates with an increase in calcium content. The Fibro-Cel carriers enable formation of 3-D architecture and mineralized tissue in vitro. © 1994 Springer-Verlag New York Inc.
Scientific Publication
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