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פותח על ידי קלירמאש פתרונות בע"מ -
Chromosome complement of the fungal plant pathogen Fusarium graminearum based on genetic and physical mapping and cytological observations
Year:
2005
Source of publication :
Genetics (מקור פרסום )
Authors :
קטן, תלמה
;
.
Volume :
171
Co-Authors:

Gale, L.R., Cereal Disease Laboratory, U.S. Department of Agriculture, Agricultural Research Service, St. Paul, MN 55108, United States
Bryant, J.D., Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108, United States
Calvo, S., Broad Institute of MIT, Harvard University, Cambridge, MA 02141, United States
Giese, H., Department of Ecology, Section of Genetics and Microbiology, Royal Veterinary and Agricultural University, Copenhagen, DK-1871 Frederiksberg C, Denmark
Katan, T., Volcani Center, 50250 Bet Dagan, Israel
O'Donnell, K., National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, Peoria, IL 61604, United States
Suga, H., Life Science Research Center, Division of Genomics Research, Gifu University, Gifu 501-1193, Japan
Taga, M., Department of Biology, Faculty of Science, Okayama University, Okayama 700-8530, Japan
Usgaard, T.R., National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, Peoria, IL 61604, United States
Ward, T.J., National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, Peoria, IL 61604, United States
Kistler, H.C., Cereal Disease Laboratory, U.S. Department of Agriculture, Agricultural Research Service, St. Paul, MN 55108, United States, Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108, United States, USDA, Cereal Disease Laboratory, University of Minnesota, 1551 Lindig St., St. Paul, MN 55108, United States

Facilitators :
From page:
985
To page:
1001
(
Total pages:
17
)
Abstract:
A genetic map of the filamentous fungus Fusarium graminearum (teleomorph: Gibberella zeae) was constructed to both validate and augment the draft whole-genome sequence assembly of strain PH-1. A mapping population was created from a cross between mutants of the sequenced strain (PH-1, NRRL 31084, originally isolated from Michigan) and a field strain from Minnesota (00-676, NRRL 34097). A total of 111 ascospore progeny were analyzed for segregation at 235 loci. Genetic markers consisted of sequence-tagged sites, primarily detected as dCAPS or CAPS (n = 131) and VNTRs (n = 31), in addition to AFLPs (n = 66) and 7 other markers. While most markers exhibited Mendelian inheritance, segregation distortion was observed for 25 predominantly clustered markers. A linkage map was generated using the Kosambi mapping function, using a LOD threshold value of 3.5. Nine linkage groups were detected, covering 1234 cM and anchoring 99.83% of the draft sequence assembly. The nine linkage groups and the 22 anchored scaffolds from the sequence assembly could be assembled into four chromosomes, leaving only five smaller scaffolds (59,630 bp total) of the nuclear DNA unanchored. A chromosome number of four was confirmed by cytological karyotyping. Further analysis of the genetic map data identified variation in recombination rate in different genomic regions that often spanned several hundred kilobases. Copyright © 2005 by the Genetics Society of America.
Note:
Related Files :
fungi
Fusarium
genetic markers
Genome
phenotype
segregation distortion
עוד תגיות
תוכן קשור
More details
DOI :
10.1534/genetics.105.044842
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21528
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:44
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Scientific Publication
Chromosome complement of the fungal plant pathogen Fusarium graminearum based on genetic and physical mapping and cytological observations
171

Gale, L.R., Cereal Disease Laboratory, U.S. Department of Agriculture, Agricultural Research Service, St. Paul, MN 55108, United States
Bryant, J.D., Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108, United States
Calvo, S., Broad Institute of MIT, Harvard University, Cambridge, MA 02141, United States
Giese, H., Department of Ecology, Section of Genetics and Microbiology, Royal Veterinary and Agricultural University, Copenhagen, DK-1871 Frederiksberg C, Denmark
Katan, T., Volcani Center, 50250 Bet Dagan, Israel
O'Donnell, K., National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, Peoria, IL 61604, United States
Suga, H., Life Science Research Center, Division of Genomics Research, Gifu University, Gifu 501-1193, Japan
Taga, M., Department of Biology, Faculty of Science, Okayama University, Okayama 700-8530, Japan
Usgaard, T.R., National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, Peoria, IL 61604, United States
Ward, T.J., National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, Peoria, IL 61604, United States
Kistler, H.C., Cereal Disease Laboratory, U.S. Department of Agriculture, Agricultural Research Service, St. Paul, MN 55108, United States, Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108, United States, USDA, Cereal Disease Laboratory, University of Minnesota, 1551 Lindig St., St. Paul, MN 55108, United States

Chromosome complement of the fungal plant pathogen Fusarium graminearum based on genetic and physical mapping and cytological observations
A genetic map of the filamentous fungus Fusarium graminearum (teleomorph: Gibberella zeae) was constructed to both validate and augment the draft whole-genome sequence assembly of strain PH-1. A mapping population was created from a cross between mutants of the sequenced strain (PH-1, NRRL 31084, originally isolated from Michigan) and a field strain from Minnesota (00-676, NRRL 34097). A total of 111 ascospore progeny were analyzed for segregation at 235 loci. Genetic markers consisted of sequence-tagged sites, primarily detected as dCAPS or CAPS (n = 131) and VNTRs (n = 31), in addition to AFLPs (n = 66) and 7 other markers. While most markers exhibited Mendelian inheritance, segregation distortion was observed for 25 predominantly clustered markers. A linkage map was generated using the Kosambi mapping function, using a LOD threshold value of 3.5. Nine linkage groups were detected, covering 1234 cM and anchoring 99.83% of the draft sequence assembly. The nine linkage groups and the 22 anchored scaffolds from the sequence assembly could be assembled into four chromosomes, leaving only five smaller scaffolds (59,630 bp total) of the nuclear DNA unanchored. A chromosome number of four was confirmed by cytological karyotyping. Further analysis of the genetic map data identified variation in recombination rate in different genomic regions that often spanned several hundred kilobases. Copyright © 2005 by the Genetics Society of America.
Scientific Publication
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