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פותח על ידי קלירמאש פתרונות בע"מ -
The use of microsatellite DNA markers for soybean genotype identification
Year:
1995
Source of publication :
Theoretical and Applied Genetics
Authors :
לביא, אורי
;
.
Volume :
90
Co-Authors:
Rongwen, J., USDA-ARS, Soybean and Alfalfa Research Laboratory, Range 1, HH-19, BARC-West, Beltsville, 20705, Maryland, United States
Akkaya, M.S., Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick, 21702, Maryland, United States
Bhagwat, A.A., Agronomy Dept., Univ. of Maryland, College Park, 20742, Maryland, United States
Lavi, U., The Volcani Center, Institute of Horticulture, ARO, Bet-Dagan, 50250, Israel
Cregan, P.B., USDA-ARS, Soybean and Alfalfa Research Laboratory, Range 1, HH-19, BARC-West, Beltsville, 20705, Maryland, United States
Facilitators :
From page:
43
To page:
48
(
Total pages:
6
)
Abstract:
Conventional morphological and pigementation traits, as well as disease resistance, have been used to distinguish the uniqueness of new soybean cultivars for purposes of plant variety protection. With increasing numbers of cultivars and a finite number of conventional characters, it has become apparent that such traits will not suffice to establish uniqueness. The objective of this work was to provide an initial evaluation of microsatellite or simple-sequence-repeat (SSR) DNA markers to develop unique DNA profiles of soybean genotypes. Microsatellites are DNA sequences such as (AT)n/(TA)n and (ATT)n/(TAA)n that are composed of tandemly repeated 2-5-basepair DNA core sequences. The DNA sequences flanking microsatellites are generally conserved allowing the selection of polymerase chain reaction (PCR) primers that will amplify the intervening SSR. Variation in the number of tandem repeats, "n", results in PCR product length differences. The SSR alleles present at three (AT)n/(TA)n and four (ATT)n/(TAA)n loci were determined in each of 96 diverse soybean genotypes. Between 11 and 26 alleles were found at each of the seven loci. Only two genotypes had identical SSR allelic profiles and these had very similar pedigrees. The gene diversity for the seven markers averaged 0.87 for all 96 genotypes and 0.74 for a subset of 26 North American cultivars. These are much higher than soybean gene diversity values obtained using RFLP markers, and are similar to the average values obtained for human microsatellite markers. SSR markers provide an excellent complement to the conventional markers that are currently used to characterize soybean genotypes. © 1995 Springer-Verlag.
Note:
Related Files :
DNA finger printing
DNA markers
Gene diversity
Glycine max
Plant variety protection
Simple sequence repeats
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00220994
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21530
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:44
Scientific Publication
The use of microsatellite DNA markers for soybean genotype identification
90
Rongwen, J., USDA-ARS, Soybean and Alfalfa Research Laboratory, Range 1, HH-19, BARC-West, Beltsville, 20705, Maryland, United States
Akkaya, M.S., Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick, 21702, Maryland, United States
Bhagwat, A.A., Agronomy Dept., Univ. of Maryland, College Park, 20742, Maryland, United States
Lavi, U., The Volcani Center, Institute of Horticulture, ARO, Bet-Dagan, 50250, Israel
Cregan, P.B., USDA-ARS, Soybean and Alfalfa Research Laboratory, Range 1, HH-19, BARC-West, Beltsville, 20705, Maryland, United States
The use of microsatellite DNA markers for soybean genotype identification
Conventional morphological and pigementation traits, as well as disease resistance, have been used to distinguish the uniqueness of new soybean cultivars for purposes of plant variety protection. With increasing numbers of cultivars and a finite number of conventional characters, it has become apparent that such traits will not suffice to establish uniqueness. The objective of this work was to provide an initial evaluation of microsatellite or simple-sequence-repeat (SSR) DNA markers to develop unique DNA profiles of soybean genotypes. Microsatellites are DNA sequences such as (AT)n/(TA)n and (ATT)n/(TAA)n that are composed of tandemly repeated 2-5-basepair DNA core sequences. The DNA sequences flanking microsatellites are generally conserved allowing the selection of polymerase chain reaction (PCR) primers that will amplify the intervening SSR. Variation in the number of tandem repeats, "n", results in PCR product length differences. The SSR alleles present at three (AT)n/(TA)n and four (ATT)n/(TAA)n loci were determined in each of 96 diverse soybean genotypes. Between 11 and 26 alleles were found at each of the seven loci. Only two genotypes had identical SSR allelic profiles and these had very similar pedigrees. The gene diversity for the seven markers averaged 0.87 for all 96 genotypes and 0.74 for a subset of 26 North American cultivars. These are much higher than soybean gene diversity values obtained using RFLP markers, and are similar to the average values obtained for human microsatellite markers. SSR markers provide an excellent complement to the conventional markers that are currently used to characterize soybean genotypes. © 1995 Springer-Verlag.
Scientific Publication
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