אסיף מאגר המחקר החקלאי

Improved 2,4-Dinitrophenvlhydrazine, Thin Layer Chromatography Methods for the Determination of Micro- and Macroamounts of Ascorbic Acid

Year:

1974

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Volume :

29

Co-Authors:

Navon, A., Division of Entomology, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan, Israel
Levinson, H.Z., Max-Planck-Institut für Verhaltensphysiologie, Seewiesen, Germany

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Abstract:

Microamounts of vitamin C could be readily determined in 20 μ1-samples using the 2.4-dinitrophenylhydrazine method together with separation by thin layer chromatography. The condensation reaction was carried out for 5 min at 100 °C on a glass fibre disc. Purification of vitamin C hydrazones was accomplished by repeated separation on TLC plates. An aqueous solution of 65% acetic acid was employed to dissolve the vitamin C hydrazones, providing maximal absorbance at 500 nm. The minimum amount detectable by this method is 0.4 μg of dehydroascorbic acid. The macrodetermination of vitamin C was improved by simplifying a previous method and employing 65% aqueous acetic acid as a solvent for the hydrazones. © 1974, Walter de Gruyter. All rights reserved.

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