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פותח על ידי קלירמאש פתרונות בע"מ -
Use of modified plum pox virus coat protein genes developed to limit heteroencapsidation-associated risks in transgenic plants
Year:
1998
Source of publication :
Journal of General Virology
Authors :
רקח, בנימין
;
.
Volume :
79
Co-Authors:
Jacquet, C., INRA, Stn. de Pathol. Veg., Centre de Recherche de Bordeaux, BP 81, 33883 Villenave d'Ornon Cedex, France
Delecolle, B., INRA, Stn. de Pathol. Veg., BP 94, 84143 Montfavet Cedex, France
Raccah, B., Department of Virology, ARO, Volcani Center, POB 6, Bet Dagan 50250, Israel
Lecoq, H., INRA, Stn. de Pathol. Veg., BP 94, 84143 Montfavet Cedex, France
Dunez, J., INRA, Stn. de Pathol. Veg., Centre de Recherche de Bordeaux, BP 81, 33883 Villenave d'Ornon Cedex, France
Ravelonandro, M., INRA, Stn. de Pathol. Veg., Centre de Recherche de Bordeaux, BP 81, 33883 Villenave d'Ornon Cedex, France
Facilitators :
From page:
1509
To page:
1517
(
Total pages:
9
)
Abstract:
Aphid transmission of a non-aphid-transmissible strain of zucchini yellow mosaic virus (ZYMV-NAT) occurs in transgenic plants expressing the plum pox potyvirus (PPV) coat protein (CP) gene. Heteroencapsidation has been shown to be responsible for this modification in the epidemiological characteristics of the infecting virus. In order to prevent this biological risk, several modified PPV CP constructs were produced that were designed to interfere with heteroencapsidation itself or to block aphid transmission of heteroencapsidated virions. These constructs were first expressed in Escherichia coli in order to check for the accumulation of pseudo-particles by electron microscopy. Virus-like particles (VLPs) were found with the full-length CP and with a PPV CP lacking the DAG amino acid triplet involved in aphid transmission. However, no VLPs were observed with CP lacking R220, Q221 or D264, amino acids known to be essential for the assembly of other potyvirus CPs. Transgenic Nicotiana benthamiana lines expressing the different PPV CP constructs were infected with ZYMV-NAT. Aphid transmission assays performed with these plants demonstrated that the strategies developed here provide an effective means of minimizing the biological risks associated with heteroencapsidation.
Note:
Related Files :
Aphididae
Mosaic virus
Plum Pox Virus
transgenic plant
virus gene
virus particle
virus transmission
Zucchini yellow mosaic virus
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21610
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:45
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Scientific Publication
Use of modified plum pox virus coat protein genes developed to limit heteroencapsidation-associated risks in transgenic plants
79
Jacquet, C., INRA, Stn. de Pathol. Veg., Centre de Recherche de Bordeaux, BP 81, 33883 Villenave d'Ornon Cedex, France
Delecolle, B., INRA, Stn. de Pathol. Veg., BP 94, 84143 Montfavet Cedex, France
Raccah, B., Department of Virology, ARO, Volcani Center, POB 6, Bet Dagan 50250, Israel
Lecoq, H., INRA, Stn. de Pathol. Veg., BP 94, 84143 Montfavet Cedex, France
Dunez, J., INRA, Stn. de Pathol. Veg., Centre de Recherche de Bordeaux, BP 81, 33883 Villenave d'Ornon Cedex, France
Ravelonandro, M., INRA, Stn. de Pathol. Veg., Centre de Recherche de Bordeaux, BP 81, 33883 Villenave d'Ornon Cedex, France
Use of modified plum pox virus coat protein genes developed to limit heteroencapsidation-associated risks in transgenic plants
Aphid transmission of a non-aphid-transmissible strain of zucchini yellow mosaic virus (ZYMV-NAT) occurs in transgenic plants expressing the plum pox potyvirus (PPV) coat protein (CP) gene. Heteroencapsidation has been shown to be responsible for this modification in the epidemiological characteristics of the infecting virus. In order to prevent this biological risk, several modified PPV CP constructs were produced that were designed to interfere with heteroencapsidation itself or to block aphid transmission of heteroencapsidated virions. These constructs were first expressed in Escherichia coli in order to check for the accumulation of pseudo-particles by electron microscopy. Virus-like particles (VLPs) were found with the full-length CP and with a PPV CP lacking the DAG amino acid triplet involved in aphid transmission. However, no VLPs were observed with CP lacking R220, Q221 or D264, amino acids known to be essential for the assembly of other potyvirus CPs. Transgenic Nicotiana benthamiana lines expressing the different PPV CP constructs were infected with ZYMV-NAT. Aphid transmission assays performed with these plants demonstrated that the strategies developed here provide an effective means of minimizing the biological risks associated with heteroencapsidation.
Scientific Publication
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