חיפוש מתקדם
Amutha, S., Department of Plant Pathology and Weed Science, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Kathiravan, K., Department of Plant Pathology and Weed Science, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel, Dept of Biotechnology, University of Madras, Guindy Campus, Chennai 600 025, India
Singer, S., Department of Plant Pathology and Weed Science, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Jashi, L., Department of Food Science, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Shomer, I., Department of Food Science, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Steinitz, B., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Gaba, V., Department of Plant Pathology and Weed Science, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Regeneration of new shoots in plant tissue culture is often associated with appearance of abnormally shaped leaves. We used the adventitious shoot regeneration response induced by decapitation (removal of all preformed shoot apical meristems, leaving a single cotyledon) of greenhouse-grown cotyledon-stage seedlings to test the hypothesis that such abnormal leaf formation is a normal regeneration progression following wounding and is not conditioned by tissue culture. To understand why shoot regeneration starts with defective organogenesis, the regeneration response was characterized by morphology and scanning electron and light microscopy in decapitated cotyledon-stage Cucurbita pepo seedlings. Several leaf primordia were observed to regenerate prior to differentiation of a de novo shoot apical meristem from dividing cells on the wound surface. Early regenerating primordia have a greatly distorted structure with dramatically altered dorsoventrality. Aberrant leaf morphogenesis in C. pepo gradually disappears as leaves eventually originate from a de novo adventitious shoot apical meristem, recovering normal phyllotaxis. Similarly, following comparable decapitation of seedlings from a number of families (Chenopodiaceae, Compositae, Convolvulaceae, Cucurbitaceae, Cruciferae, Fabaceae, Malvaceae, Papaveraceae, and Solanaceae) of several dicotyledonous clades (Ranunculales, Caryophyllales, Asterids, and Rosids), stems are regenerated bearing abnormal leaves; the normal leaf shape is gradually recovered. Some of the transient leaf developmental defects observed are similar to responses to mutations in leaf shape or shoot apical meristem function. Many species temporarily express this leaf development pathway, which is manifest in exceptional circumstances such as during recovery from excision of all preformed shoot meristems of a seedling. © The Society for In Vitro Biology 2009.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Adventitious shoot formation in decapitated dicotyledonous seedlings starts with regeneration of abnormal leaves from cells not located in a shoot apical meristem
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Amutha, S., Department of Plant Pathology and Weed Science, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Kathiravan, K., Department of Plant Pathology and Weed Science, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel, Dept of Biotechnology, University of Madras, Guindy Campus, Chennai 600 025, India
Singer, S., Department of Plant Pathology and Weed Science, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Jashi, L., Department of Food Science, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Shomer, I., Department of Food Science, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Steinitz, B., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Gaba, V., Department of Plant Pathology and Weed Science, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Adventitious shoot formation in decapitated dicotyledonous seedlings starts with regeneration of abnormal leaves from cells not located in a shoot apical meristem
Regeneration of new shoots in plant tissue culture is often associated with appearance of abnormally shaped leaves. We used the adventitious shoot regeneration response induced by decapitation (removal of all preformed shoot apical meristems, leaving a single cotyledon) of greenhouse-grown cotyledon-stage seedlings to test the hypothesis that such abnormal leaf formation is a normal regeneration progression following wounding and is not conditioned by tissue culture. To understand why shoot regeneration starts with defective organogenesis, the regeneration response was characterized by morphology and scanning electron and light microscopy in decapitated cotyledon-stage Cucurbita pepo seedlings. Several leaf primordia were observed to regenerate prior to differentiation of a de novo shoot apical meristem from dividing cells on the wound surface. Early regenerating primordia have a greatly distorted structure with dramatically altered dorsoventrality. Aberrant leaf morphogenesis in C. pepo gradually disappears as leaves eventually originate from a de novo adventitious shoot apical meristem, recovering normal phyllotaxis. Similarly, following comparable decapitation of seedlings from a number of families (Chenopodiaceae, Compositae, Convolvulaceae, Cucurbitaceae, Cruciferae, Fabaceae, Malvaceae, Papaveraceae, and Solanaceae) of several dicotyledonous clades (Ranunculales, Caryophyllales, Asterids, and Rosids), stems are regenerated bearing abnormal leaves; the normal leaf shape is gradually recovered. Some of the transient leaf developmental defects observed are similar to responses to mutations in leaf shape or shoot apical meristem function. Many species temporarily express this leaf development pathway, which is manifest in exceptional circumstances such as during recovery from excision of all preformed shoot meristems of a seedling. © The Society for In Vitro Biology 2009.
Scientific Publication
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