Bone-related matrix proteins expression in vitro and in vivo by marrow stromal cell line

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Bone-related matrix proteins expression in vitro and in vivo by marrow stromal cell line

Year: 

1994

Source of publication :

Tissue and Cell

Authors :

שמאי, אבי

.

Volume :

Co-Authors: 

Benayahu, D., Department of Histology and Cell Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel-Aviv, 69978, Israel
Gurevitz, O.A., Department of Bone Marrow Transplantation, Hadassah Ein-Kerem, The Hebrew University of Jerusalem, Jerusalem, Israel
Shamay, A., Department of Histology and Cell Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel-Aviv, 69978, Israel

From page: 

661

To page: 

666

(

Total pages: 

6

)

Link :

Abstract: 

MBA-1, a bone marrow stroma-derived cell line, was transplanted in an ectopic site and formed endochondral bone. The ossicle developed through stages of cell proliferation, differentiated into a zone of hypertrophy and formed a chondroid-like area which further developed to primary mineralized bone. We explored the expression of various matrix proteins by MBA-1 cells in vitro and in the ossicle formed in vivo. MBA-1 cells constitutively expressed mRNAs encoding for collagen I, non-collagenous proteins and alkaline phosphatase. RNA extracted from the ossicle formed by these cells was expressed in a different pattern. The in vivo maturation of MBA-1 cells was accompanied by low expression of mRNA for procollagen α2(I) and a marked increase in osteonectin and osteopontin mRNA levels. Thus, the ability to follow expression of these genes through bone formation in vivo has been demonstrated. © 1994.