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Tissue and Cell
Benayahu, D., Department of Histology and Cell Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel-Aviv, 69978, Israel
Gurevitz, O.A., Department of Bone Marrow Transplantation, Hadassah Ein-Kerem, The Hebrew University of Jerusalem, Jerusalem, Israel
Shamay, A., Department of Histology and Cell Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel-Aviv, 69978, Israel
MBA-1, a bone marrow stroma-derived cell line, was transplanted in an ectopic site and formed endochondral bone. The ossicle developed through stages of cell proliferation, differentiated into a zone of hypertrophy and formed a chondroid-like area which further developed to primary mineralized bone. We explored the expression of various matrix proteins by MBA-1 cells in vitro and in the ossicle formed in vivo. MBA-1 cells constitutively expressed mRNAs encoding for collagen I, non-collagenous proteins and alkaline phosphatase. RNA extracted from the ossicle formed by these cells was expressed in a different pattern. The in vivo maturation of MBA-1 cells was accompanied by low expression of mRNA for procollagen α2(I) and a marked increase in osteonectin and osteopontin mRNA levels. Thus, the ability to follow expression of these genes through bone formation in vivo has been demonstrated. © 1994.
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תנאי שימוש
Bone-related matrix proteins expression in vitro and in vivo by marrow stromal cell line
26
Benayahu, D., Department of Histology and Cell Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel-Aviv, 69978, Israel
Gurevitz, O.A., Department of Bone Marrow Transplantation, Hadassah Ein-Kerem, The Hebrew University of Jerusalem, Jerusalem, Israel
Shamay, A., Department of Histology and Cell Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel-Aviv, 69978, Israel
Bone-related matrix proteins expression in vitro and in vivo by marrow stromal cell line
MBA-1, a bone marrow stroma-derived cell line, was transplanted in an ectopic site and formed endochondral bone. The ossicle developed through stages of cell proliferation, differentiated into a zone of hypertrophy and formed a chondroid-like area which further developed to primary mineralized bone. We explored the expression of various matrix proteins by MBA-1 cells in vitro and in the ossicle formed in vivo. MBA-1 cells constitutively expressed mRNAs encoding for collagen I, non-collagenous proteins and alkaline phosphatase. RNA extracted from the ossicle formed by these cells was expressed in a different pattern. The in vivo maturation of MBA-1 cells was accompanied by low expression of mRNA for procollagen α2(I) and a marked increase in osteonectin and osteopontin mRNA levels. Thus, the ability to follow expression of these genes through bone formation in vivo has been demonstrated. © 1994.
Scientific Publication
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