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פותח על ידי קלירמאש פתרונות בע"מ -
Development of microsatellite markers from the transcriptome of Erysiphe necator for analysing population structure in North America and Europe
Year:
2012
Source of publication :
Plant Pathology
Authors :
פרנקל, עומר
;
.
Volume :
61
Co-Authors:
Frenkel, O., Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, United States, Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, POB 6, Bet-Dagan 50250, Israel
Portillo, I., Dipartimento di Protezione e Valorizzazione Agro-Alimentare, Università degli Studi di Bologna, Viale Fanin 46, 40100 Bologna, Italy
Brewer, M.T., Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, United States, Department of Plant Pathology, University of Georgia, Athens, GA 30602, United States
Péros, J.P., Institut National de la Recherche Agronomique, UMR 1334, Equipe DAVEM, 2 place Viala, 34060 Montpellier, France
Cadle-Davidson, L., United States Department of Agriculture-Agricultural Research Service, Grape Genetics Research Unit, Geneva, NY 14456, United States
Milgroom, M.G., Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, United States
Facilitators :
From page:
106
To page:
119
(
Total pages:
14
)
Abstract:
Transcriptome sequences of the grape powdery mildew fungus Erysiphe necator were used to develop microsatellite markers (EST-SSRs) to study its relatively unexplored population structure in its centre of diversity in eastern North America. Screening the transcriptome sequences revealed 116 contigs with candidate microsatellites, from which 11 polymorphic microsatellite markers were developed from 31 markers tested. Eight of these markers were used to genotype isolates from different regions and hosts in the eastern USA and compare them to samples from southern France and Italy. Genetic diversity in the eastern USA is much greater than in Europe. Bayesian cluster analyses showed that 10 isolates from North America have high affinities with, but differ from, European group A; these are referred to as A-like isolates. No isolates with close affinity to European group B were found in the eastern USA. Bayesian analyses also detected genetic differentiation between isolates from Vitis rotundifolia and isolates from other Vitis hosts. Genetic differentiation detected between the northeastern and southeastern USA was mostly attributable to the A-like isolates in the southeast, which are significantly more aggressive than the other populations. This research demonstrates that transcriptome sequencing of fungal pathogens is useful for developing genetic markers in protein-coding regions and highlights the role of these markers in population biology studies of E. necator. © 2011 The Authors. Plant Pathology © 2011 BSPP.
Note:
Related Files :
France
fungi
grape
Italy
Necator
Sampling
Vitaceae
Vitis
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1365-3059.2011.02502.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21703
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:46
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Scientific Publication
Development of microsatellite markers from the transcriptome of Erysiphe necator for analysing population structure in North America and Europe
61
Frenkel, O., Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, United States, Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, POB 6, Bet-Dagan 50250, Israel
Portillo, I., Dipartimento di Protezione e Valorizzazione Agro-Alimentare, Università degli Studi di Bologna, Viale Fanin 46, 40100 Bologna, Italy
Brewer, M.T., Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, United States, Department of Plant Pathology, University of Georgia, Athens, GA 30602, United States
Péros, J.P., Institut National de la Recherche Agronomique, UMR 1334, Equipe DAVEM, 2 place Viala, 34060 Montpellier, France
Cadle-Davidson, L., United States Department of Agriculture-Agricultural Research Service, Grape Genetics Research Unit, Geneva, NY 14456, United States
Milgroom, M.G., Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, United States
Development of microsatellite markers from the transcriptome of Erysiphe necator for analysing population structure in North America and Europe
Transcriptome sequences of the grape powdery mildew fungus Erysiphe necator were used to develop microsatellite markers (EST-SSRs) to study its relatively unexplored population structure in its centre of diversity in eastern North America. Screening the transcriptome sequences revealed 116 contigs with candidate microsatellites, from which 11 polymorphic microsatellite markers were developed from 31 markers tested. Eight of these markers were used to genotype isolates from different regions and hosts in the eastern USA and compare them to samples from southern France and Italy. Genetic diversity in the eastern USA is much greater than in Europe. Bayesian cluster analyses showed that 10 isolates from North America have high affinities with, but differ from, European group A; these are referred to as A-like isolates. No isolates with close affinity to European group B were found in the eastern USA. Bayesian analyses also detected genetic differentiation between isolates from Vitis rotundifolia and isolates from other Vitis hosts. Genetic differentiation detected between the northeastern and southeastern USA was mostly attributable to the A-like isolates in the southeast, which are significantly more aggressive than the other populations. This research demonstrates that transcriptome sequencing of fungal pathogens is useful for developing genetic markers in protein-coding regions and highlights the role of these markers in population biology studies of E. necator. © 2011 The Authors. Plant Pathology © 2011 BSPP.
Scientific Publication
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