אסיף מאגר המחקר החקלאי

LaeA regulation of secondary metabolism modulates virulence in Penicillium expansum and is mediated by sucrose

Year:

2016

Source of publication :

Authors :

Volume :

18

Co-Authors:

Chen, Y., Key Laboratory of Plant ResourcesInstitute of Botany, Chinese Academy of SciencesBeijing100093 China
Luo, X., Department of Plant PathologyUniversity of Wisconsin - MadisonMadison 53706-1598WI USA
Tannous, J., Department of Medical Microbiology and ImmunologyUniversity of Wisconsin - MadisonMadison 53706-1598WI USA
Dubey, A., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan50250 Israel
Tian, S., Key Laboratory of Plant ResourcesInstitute of Botany, Chinese Academy of SciencesBeijing100093 China
Li, B., Key Laboratory of Plant ResourcesInstitute of Botany, Chinese Academy of SciencesBeijing100093 China
Keller, N., Department of Plant PathologyUniversity of Wisconsin - MadisonMadison 53706-1598WI USA, Department of Medical Microbiology and ImmunologyUniversity of Wisconsin - MadisonMadison 53706-1598WI USA, Department of BacteriologyUniversity of Wisconsin - MadisonMadison 53706-1598WI USA
Prusky, D., Department of Postharvest Science of Fresh ProduceAgricultural Research Organization, The Volcani CenterBet Dagan50250 Israel

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Abstract:

Penicillium expansum, the causal agent of blue mould rot, is a critical health concern because of the production of the mycotoxin patulin in colonized apple fruit tissue. Although patulin is produced by many Penicillium species, the factor(s) activating its biosynthesis are not clear. Sucrose, a key sugar component of apple fruit, was found to modulate patulin accumulation in a dose-responsive pattern. An increase in sucrose culture amendment from 15 to 175 mm decreased both patulin accumulation and expression of the global regulator laeA by 175- and five-fold, respectively, whilst increasing expression of the carbon catabolite repressor creA. LaeA was found to regulate several secondary metabolite genes, including the patulin gene cluster and concomitant patulin synthesis in vitro. Virulence studies of ΔlaeA mutants of two geographically distant P. expansum isolates (Pe-21 from Israel and Pe-T01 from China) showed differential reduction in disease severity in freshly harvested fruit, ranging from no reduction for Ch-Pe-T01 strains to 15%-25% reduction for both strains in mature fruit, with the ΔlaeA strains of Is-Pe-21 always showing a greater loss in virulence. The results suggest the importance of abiotic factors in LaeA regulation of patulin and other secondary metabolites that contribute to pathogenicity. © 2016 BSPP AND JOHN WILEY & SONS LTD.

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