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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Chlorophyll breakdown by chlorophyllase: Isolation and functional expression of the Chlase1 gene from ethylene-treated Citrus fruit and its regulation during development
Year:
1999
Source of publication :
Plant Journal
Authors :
איל, יורם
;
.
הולנד, דורון
;
.
Volume :
20
Co-Authors:
Jacob-Wilk, D., Kennedy-Leigh Ctr. for Hort. Res., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, Rehovot 76100, Israel, Dept. Fruit-Tree Breed. Molec. G., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Holland, D., Dept. Fruit-Tree Breed. Molec. G., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Goldschmidt, E.E., Kennedy-Leigh Ctr. for Hort. Res., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, Rehovot 76100, Israel
Riov, J., Kennedy-Leigh Ctr. for Hort. Res., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, Rehovot 76100, Israel
Eyal, Y., Dept. Fruit-Tree Breed. Molec. G., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
653
To page:
661
(
Total pages:
9
)
Abstract:
We report on the isolation, functional expression and characterization of a cDNA encoding chlorophyllase, the enzyme catalyzing the first step in the chlorophyll breakdown pathway. The Chlase1 cDNA from Valencia Orange (Citrus sinensis cv. Valencia) was obtained by RT-PCR using degenerate primers based on the amino acid sequence of the previously purified protein. Chlase1 encodes a protein of 329 amino acids, including sequence domain characterizing serine-lipases and a putative chloroplast-directing transit peptide. The Chlase1 gene encodes an active chlorophyllase enzyme which catalyzes the dephytylation of chlorophyll as shown by in vitro recombinant enzyme assays. Chlorophyllase expression at the transcript level in Valencia orange peel was found to be low and constitutive during natural fruit development without significant increase towards color-break and ripening. However, ethylene treatment induced an increase in chlorophyllase transcript at all stages of development. An enhanced response to ethylene treatment was observed during the months of October and November, corresponding to the time of natural color-break. The senescence-delaying regulator gibberellin-A3 (GA3) inhibited the effect of ethylene on chlorophyllase transcript accumulation. The data presented suggest that chlorophyllase may not be the regulator of chlorophyll breakdown during natural fruit ripening but is consistent with the notion that chlorophyll is gradually degraded during ripening due to a negative balance between synthesis and breakdown. According to this model, exogenous application of ethylene accelerates chlorophyll breakdown due to increased de novo synthesis of chlorophyllase. Further experimentation on the regulation and role of chlorophyllase in planta will be facilitated by the gene tools established in this work.
Note:
Related Files :
Base Sequence
chlorophyll
chlorophyllase
ethylene
gibberellins
Molecular Sequence Data
ripening
עוד תגיות
תוכן קשור
More details
DOI :
10.1046/j.1365-313X.1999.00637.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21858
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:47
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Chlorophyll breakdown by chlorophyllase: Isolation and functional expression of the Chlase1 gene from ethylene-treated Citrus fruit and its regulation during development
20
Jacob-Wilk, D., Kennedy-Leigh Ctr. for Hort. Res., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, Rehovot 76100, Israel, Dept. Fruit-Tree Breed. Molec. G., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Holland, D., Dept. Fruit-Tree Breed. Molec. G., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Goldschmidt, E.E., Kennedy-Leigh Ctr. for Hort. Res., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, Rehovot 76100, Israel
Riov, J., Kennedy-Leigh Ctr. for Hort. Res., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, Rehovot 76100, Israel
Eyal, Y., Dept. Fruit-Tree Breed. Molec. G., Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan, 50250, Israel
Chlorophyll breakdown by chlorophyllase: Isolation and functional expression of the Chlase1 gene from ethylene-treated Citrus fruit and its regulation during development
We report on the isolation, functional expression and characterization of a cDNA encoding chlorophyllase, the enzyme catalyzing the first step in the chlorophyll breakdown pathway. The Chlase1 cDNA from Valencia Orange (Citrus sinensis cv. Valencia) was obtained by RT-PCR using degenerate primers based on the amino acid sequence of the previously purified protein. Chlase1 encodes a protein of 329 amino acids, including sequence domain characterizing serine-lipases and a putative chloroplast-directing transit peptide. The Chlase1 gene encodes an active chlorophyllase enzyme which catalyzes the dephytylation of chlorophyll as shown by in vitro recombinant enzyme assays. Chlorophyllase expression at the transcript level in Valencia orange peel was found to be low and constitutive during natural fruit development without significant increase towards color-break and ripening. However, ethylene treatment induced an increase in chlorophyllase transcript at all stages of development. An enhanced response to ethylene treatment was observed during the months of October and November, corresponding to the time of natural color-break. The senescence-delaying regulator gibberellin-A3 (GA3) inhibited the effect of ethylene on chlorophyllase transcript accumulation. The data presented suggest that chlorophyllase may not be the regulator of chlorophyll breakdown during natural fruit ripening but is consistent with the notion that chlorophyll is gradually degraded during ripening due to a negative balance between synthesis and breakdown. According to this model, exogenous application of ethylene accelerates chlorophyll breakdown due to increased de novo synthesis of chlorophyllase. Further experimentation on the regulation and role of chlorophyllase in planta will be facilitated by the gene tools established in this work.
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