Parsons, E.P., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Popopvsky, S., Agricultural Research Organization, Volcani Center (ARO), Bet Dagan, 50250, Israel
Lohrey, G.T., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Lü, S., Center for Plant Stress Genomics and Technology, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia
Alkalai-Tuvia, S., Agricultural Research Organization, Volcani Center (ARO), Bet Dagan, 50250, Israel
Perzelan, Y., Agricultural Research Organization, Volcani Center (ARO), Bet Dagan, 50250, Israel
Paran, I., Agricultural Research Organization, Volcani Center (ARO), Bet Dagan, 50250, Israel
Fallik, E., Agricultural Research Organization, Volcani Center (ARO), Bet Dagan, 50250, Israel
Jenks, M.A., US Arid Land Agricultural Research Center, Maricopa, AZ 85138, United States

To understand the role of fruit cuticle lipid composition in fruit water loss, an advanced backcross population, the BC2F2, was created between the Capsicum annuum (PI1154) and the Capsicum chinense (USDA162), which have high and low post-harvest water loss rates, respectively. Besides dramatic differences in fruit water loss, preliminary studies also revealed that these parents exhibited significant differences in both the amount and composition of their fruit cuticle. Cuticle analysis of the BC2F2 fruit revealed that although water loss rate was not strongly associated with the total surface wax amount, there were significant correlations between water loss rate and cuticle composition. We found a positive correlation between water loss rate and the amount of total triterpenoid plus sterol compounds, and negative correlations between water loss and the alkane to triterpenoid plus sterol ratio. We also report negative correlations between water loss rate and the proportion of both alkanes and aliphatics to total surface wax amount. For the first time, we report significant correlations between water loss and cutin monomer composition. We found positive associations of water loss rate with the total cutin, total C16 monomers and 16-dihydroxy hexadecanoic acid. Our results support the hypothesis that simple straight-chain aliphatic cuticle constituents form more impermeable cuticular barriers than more complex isoprenoid-based compounds. These results shed new light on the biochemical basis for cuticle involvement in fruit water loss. © 2012 Physiologia Plantarum.