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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Halofuginone reduces the occurrence of renal fibrosis in 5/6 nephrectomized rats
Year:
2007
Source of publication :
Israel Medical Association Journal
Authors :
פינס, מרק
;
.
Volume :
9
Co-Authors:
Benchetrit, S., Department of Nephrology and Hypertension, Sapir Medical Center, Kfar Saba 44281, Israel
Yarkoni, S., Collgard Biopharmaceuticals Ltd., Tel Aviv, Israel
Rathaus, M., Department of Nephrology and Hypertension, Sapir Medical Center, Kfar Saba 44281, Israel
Pines, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Beit Dagan, Israel
Rashid, G., Department of Nephrology and Hypertension, Sapir Medical Center, Kfar Saba 44281, Israel
Bernheim, J., Department of Pathology, Sapir Medical Center, Kfar Saba 44281, Israel, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel
Bernheim, J., Department of Nephrology and Hypertension, Sapir Medical Center, Kfar Saba 44281, Israel
Facilitators :
From page:
30
To page:
34
(
Total pages:
5
)
Abstract:
Background: Halofuginone is a novel antifibrotic agent that can reverse the fibrotic process by specific inhibition of collagen type I synthesis. Objectives: To evaluate the effect of Halo on the development of glomerulosclerosis and interstitial fibrosis in the 5/6 nephrectomy rat model. Methods: Male Wistar rats were assigned to undergo 5/6 NX or sham operation, and then divided into three groups: 5/6 NX rats (NX-Halo and NX-Control) and sham. Systolic blood pressure, proteinuria and body weight were determined every 2 weeks. At sacrifice (10 weeks) creatinine clearance was evaluated and remnant kidneys removed for histologic examination, sirius red staining and in situ hybridization. Results: Systolic blood pressure increased progressively in both 5/6 NX groups. Halo slowed the increase in proteinuria in 5/6 NX rats. As expected, creatinine clearance was lower in 5/6 NX groups when compared to sham rats. Creatinine clearance was significantly higher in the NX-Halo group at the end of the study period. Histologic examination by light microscopy showed significanty less severe interstitial fibrosis and glomerulosclerosis in Halo-treated rats. The increase in Collagen α1 (I) gene expression and collagen staining after nephrectomy was almost completely abolished by Halo. Conclusions: Halofuginone reduced proteinuria as well as the severity of interstitial fibrosis and glomerulosclerosis in 5/6 NX rats. The renal beneficial effect of Halo was also demonstrated by the blunted decrease in creatinine clearance observed in the treated animals.
Note:
Related Files :
animal experiment
animal model
Animals
animal tissue
Fibrosis
gene expression
Male
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21914
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:47
Scientific Publication
Halofuginone reduces the occurrence of renal fibrosis in 5/6 nephrectomized rats
9
Benchetrit, S., Department of Nephrology and Hypertension, Sapir Medical Center, Kfar Saba 44281, Israel
Yarkoni, S., Collgard Biopharmaceuticals Ltd., Tel Aviv, Israel
Rathaus, M., Department of Nephrology and Hypertension, Sapir Medical Center, Kfar Saba 44281, Israel
Pines, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Beit Dagan, Israel
Rashid, G., Department of Nephrology and Hypertension, Sapir Medical Center, Kfar Saba 44281, Israel
Bernheim, J., Department of Pathology, Sapir Medical Center, Kfar Saba 44281, Israel, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel
Bernheim, J., Department of Nephrology and Hypertension, Sapir Medical Center, Kfar Saba 44281, Israel
Halofuginone reduces the occurrence of renal fibrosis in 5/6 nephrectomized rats
Background: Halofuginone is a novel antifibrotic agent that can reverse the fibrotic process by specific inhibition of collagen type I synthesis. Objectives: To evaluate the effect of Halo on the development of glomerulosclerosis and interstitial fibrosis in the 5/6 nephrectomy rat model. Methods: Male Wistar rats were assigned to undergo 5/6 NX or sham operation, and then divided into three groups: 5/6 NX rats (NX-Halo and NX-Control) and sham. Systolic blood pressure, proteinuria and body weight were determined every 2 weeks. At sacrifice (10 weeks) creatinine clearance was evaluated and remnant kidneys removed for histologic examination, sirius red staining and in situ hybridization. Results: Systolic blood pressure increased progressively in both 5/6 NX groups. Halo slowed the increase in proteinuria in 5/6 NX rats. As expected, creatinine clearance was lower in 5/6 NX groups when compared to sham rats. Creatinine clearance was significantly higher in the NX-Halo group at the end of the study period. Histologic examination by light microscopy showed significanty less severe interstitial fibrosis and glomerulosclerosis in Halo-treated rats. The increase in Collagen α1 (I) gene expression and collagen staining after nephrectomy was almost completely abolished by Halo. Conclusions: Halofuginone reduced proteinuria as well as the severity of interstitial fibrosis and glomerulosclerosis in 5/6 NX rats. The renal beneficial effect of Halo was also demonstrated by the blunted decrease in creatinine clearance observed in the treated animals.
Scientific Publication
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