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פותח על ידי קלירמאש פתרונות בע"מ -
Identification of a grapefruit cDNA belonging to a unique class of citrus dehydrins and characterization of its expression patterns under temperature stress conditions
Year:
2002
Source of publication :
Physiologia Plantarum
Authors :
לוריא, סוזן
;
.
פבונצ'לו, דוד
;
.
פורת, רון
;
.
Volume :
115
Co-Authors:
Porat, R., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Pavoncello, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Lurie, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Mccollum, T.G., USDA, USHRL, 2001 S. Rock Road, Fort Pierce, FL 34945, United States
Facilitators :
From page:
598
To page:
603
(
Total pages:
6
)
Abstract:
Citrus fruits are sensitive to low temperatures and this often results in the development of chilling injuries during postharvest storage. In order to gain more insight into the molecular mechanisms involved in the acquisition of fruit chilling tolerance, we initiated a grapefruit (Citrus paradisi, cv. Marsh Seedless) flavedo cDNA sequencing project and used it to identify a cDNA similar to other Poncirus trifoliata and Citrus unshiu dehydrin genes reported to be responsive to low temperatures. The grapefruit dehydrin cDNA, designated cor15, encodes a predicted polypeptide of 15.1 kDa, that is almost completely identical with other reported citrus dehydrin proteins, except that it contains two large amino acid repeats, whereas P.trifoliata COR11 has only one such repeat and P.trifoliata COR19 and C.unshiu COR19 have three repeats. Together, the various grapefruit, P.trifoliata and C.unshiu dehydrins form a closely related and unique dehydrin gene family that differs from most other plant dehydrins in having an unusual K-segment similar to that of gymnosperms and in having a serine cluster (S-segment) at an unusual position at the carboxy-terminus. The grapefruit cor15 gene is consistently expressed in the fruit peel tissue at harvest, but its message levels dramatically decrease during storage at 2°C. However, a pre-storage hot water treatment, which enhances fruit chilling tolerance, elicited retention of the constant level of cor15 gene expression during cold storage and eliminated its decline. The hot water treatment had no inductive effect on cor15 gene expression when the fruit were held at non-chilling temperatures. The effects of other stresses, such as exposure to ethylene, UV irradiation and wounding, on cor15 gene expression, were temporary and persisted for 1-2 days after the treatments.
Note:
Related Files :
Amino Acids
Citrus unshiu
Citrus x paradisi
DNA
ethylene
Fruits
Genes
Gymnospermae
harvesting
עוד תגיות
תוכן קשור
More details
DOI :
10.1034/j.1399-3054.2002.1150414.x
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21951
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:48
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Scientific Publication
Identification of a grapefruit cDNA belonging to a unique class of citrus dehydrins and characterization of its expression patterns under temperature stress conditions
115
Porat, R., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Pavoncello, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Lurie, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Mccollum, T.G., USDA, USHRL, 2001 S. Rock Road, Fort Pierce, FL 34945, United States
Identification of a grapefruit cDNA belonging to a unique class of citrus dehydrins and characterization of its expression patterns under temperature stress conditions
Citrus fruits are sensitive to low temperatures and this often results in the development of chilling injuries during postharvest storage. In order to gain more insight into the molecular mechanisms involved in the acquisition of fruit chilling tolerance, we initiated a grapefruit (Citrus paradisi, cv. Marsh Seedless) flavedo cDNA sequencing project and used it to identify a cDNA similar to other Poncirus trifoliata and Citrus unshiu dehydrin genes reported to be responsive to low temperatures. The grapefruit dehydrin cDNA, designated cor15, encodes a predicted polypeptide of 15.1 kDa, that is almost completely identical with other reported citrus dehydrin proteins, except that it contains two large amino acid repeats, whereas P.trifoliata COR11 has only one such repeat and P.trifoliata COR19 and C.unshiu COR19 have three repeats. Together, the various grapefruit, P.trifoliata and C.unshiu dehydrins form a closely related and unique dehydrin gene family that differs from most other plant dehydrins in having an unusual K-segment similar to that of gymnosperms and in having a serine cluster (S-segment) at an unusual position at the carboxy-terminus. The grapefruit cor15 gene is consistently expressed in the fruit peel tissue at harvest, but its message levels dramatically decrease during storage at 2°C. However, a pre-storage hot water treatment, which enhances fruit chilling tolerance, elicited retention of the constant level of cor15 gene expression during cold storage and eliminated its decline. The hot water treatment had no inductive effect on cor15 gene expression when the fruit were held at non-chilling temperatures. The effects of other stresses, such as exposure to ethylene, UV irradiation and wounding, on cor15 gene expression, were temporary and persisted for 1-2 days after the treatments.
Scientific Publication
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