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פותח על ידי קלירמאש פתרונות בע"מ -
Potato skin proteome is enriched with plant defence components
Year:
2008
Source of publication :
Journal of Experimental Botany
Authors :
בראל, גילי
;
.
גינזברג, עידית
;
.
Volume :
59
Co-Authors:
Barel, G., Institute of Plant Sciences, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Ginzberg, I., Institute of Plant Sciences, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
3347
To page:
3357
(
Total pages:
11
)
Abstract:
Periderm is a tissue of secondary origin that replaces damaged epidermis. It can be found in underground plant organs, as an above-ground tissue of woody species (cork), and as a wound-healing tissue. Its outer layers are composed of phellem cells with suberized walls that constitute a protective barrier, preventing pathogen invasion and fluid loss. In potato, a model for periderm studies, periderm tissue replaces the epidermis early in tuber development and the suberized phellems constitute the tuber's skin. To identify factors involved in phellem/skin development and that play a role in its defensive characteristics, two-dimensional gel electrophoresis was used to compare the skin and parenchymatic flesh proteomes of young developing tubers. Proteins exhibiting differentially high signal intensity in the skin were sorted by functional categories. As expected, the differential skin proteome was enriched in proteins whose activity is characteristic of actively dividing tissues such as cell proliferation, C1 metabolism, and the oxidative respiratory chain. Interestingly, the major functional category consisted of proteins (63%) involved in plant defence responses to biotic and abiotic stresses. This group included three isozymes of caffeoyl-CoA O-methyltransferase and five isozymes of peroxidase that may play a role in suberization processes. The differential expression of these proteins in the skin was further verified by RT-PCR of their corresponding transcripts in skin and tuber flesh samples. The results presented here shed light on the early events in skin development and further expand the concept of the periderm as a protective tissue containing an array of plant defence components. © 2008 The Author(s).
Note:
Related Files :
Genetics
Growth, Development and Aging
metabolism
proteomics
Solanum tuberosum
עוד תגיות
תוכן קשור
More details
DOI :
10.1093/jxb/ern184
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21975
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:48
Scientific Publication
Potato skin proteome is enriched with plant defence components
59
Barel, G., Institute of Plant Sciences, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Ginzberg, I., Institute of Plant Sciences, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Potato skin proteome is enriched with plant defence components
Periderm is a tissue of secondary origin that replaces damaged epidermis. It can be found in underground plant organs, as an above-ground tissue of woody species (cork), and as a wound-healing tissue. Its outer layers are composed of phellem cells with suberized walls that constitute a protective barrier, preventing pathogen invasion and fluid loss. In potato, a model for periderm studies, periderm tissue replaces the epidermis early in tuber development and the suberized phellems constitute the tuber's skin. To identify factors involved in phellem/skin development and that play a role in its defensive characteristics, two-dimensional gel electrophoresis was used to compare the skin and parenchymatic flesh proteomes of young developing tubers. Proteins exhibiting differentially high signal intensity in the skin were sorted by functional categories. As expected, the differential skin proteome was enriched in proteins whose activity is characteristic of actively dividing tissues such as cell proliferation, C1 metabolism, and the oxidative respiratory chain. Interestingly, the major functional category consisted of proteins (63%) involved in plant defence responses to biotic and abiotic stresses. This group included three isozymes of caffeoyl-CoA O-methyltransferase and five isozymes of peroxidase that may play a role in suberization processes. The differential expression of these proteins in the skin was further verified by RT-PCR of their corresponding transcripts in skin and tuber flesh samples. The results presented here shed light on the early events in skin development and further expand the concept of the periderm as a protective tissue containing an array of plant defence components. © 2008 The Author(s).
Scientific Publication
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