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פותח על ידי קלירמאש פתרונות בע"מ -
A simple procedure for the extraction of double-stranded RNA from virus-infected plants
Year:
1983
Source of publication :
Journal of Virological Methods
Authors :
בר-יוסף, משה
;
.
מוסקוביץ, מירה
;
.
רוזנר, אריה
;
.
Volume :
6
Co-Authors:
Bar-Joseph, M., Virus Laboratory, Volconi Center, A.R.O., Bet Dagan, Israel
Rosner, A., Virus Laboratory, Volconi Center, A.R.O., Bet Dagan, Israel
Moscovitz, M., Virus Laboratory, Volconi Center, A.R.O., Bet Dagan, Israel
Hull, R., Department of Virus Research, John Innes Institute, Colney Lane, Norwich, United Kingdom
Facilitators :
From page:
1
To page:
8
(
Total pages:
8
)
Abstract:
A simple procedure for the isolation of double-stranded (ds) RNA from virus-infected plants is described. The method is based on grinding plant tissue in 4% p-aminosalicylic acid and recovery of ds RNA by phenol extraction and precipitation with 30% ethanol. The presence of both negative and positive virus RNA strands in RNA fractionated in agarose gels was verified by Northern blot hybridization with polynucleotide kinase labelled genomic RNA or complementary DNA (cDNA) probes. The procedure enabled detection of three major ds RNA species (MWs 4.2, 1.05 and 0.48 × 106) and at least 4 minor bands with estimated MWs of 3.5, 2.5, 2.2 and 2.0 × 106 in Nicotiana tabacum plants systemically infected with tobacco mosaic virus (TMV). Cucumber mosaic virus (CMV)-infected Pachystachys coccinea plants contained 2 minor bands of MWs 0.49 and 0.35 × 106 in addition to the previously described 4 major ds RNAs and ds CARNA 5 (MW 0.22 × 106). The patterns of ds RNA are useful for diagnosing natural infections of CMV and TMV in N. glauca plants and of citrus tristeza virus in Citrus spp. © 1983.
Note:
Related Files :
double-stranded RNA
higher plant
methodology
Plants
Tobacco mosaic virus
tobacco mosaic virus double-stranded RNA
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0166-0934(83)90062-9
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
21982
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:48
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Scientific Publication
A simple procedure for the extraction of double-stranded RNA from virus-infected plants
6
Bar-Joseph, M., Virus Laboratory, Volconi Center, A.R.O., Bet Dagan, Israel
Rosner, A., Virus Laboratory, Volconi Center, A.R.O., Bet Dagan, Israel
Moscovitz, M., Virus Laboratory, Volconi Center, A.R.O., Bet Dagan, Israel
Hull, R., Department of Virus Research, John Innes Institute, Colney Lane, Norwich, United Kingdom
A simple procedure for the extraction of double-stranded RNA from virus-infected plants
A simple procedure for the isolation of double-stranded (ds) RNA from virus-infected plants is described. The method is based on grinding plant tissue in 4% p-aminosalicylic acid and recovery of ds RNA by phenol extraction and precipitation with 30% ethanol. The presence of both negative and positive virus RNA strands in RNA fractionated in agarose gels was verified by Northern blot hybridization with polynucleotide kinase labelled genomic RNA or complementary DNA (cDNA) probes. The procedure enabled detection of three major ds RNA species (MWs 4.2, 1.05 and 0.48 × 106) and at least 4 minor bands with estimated MWs of 3.5, 2.5, 2.2 and 2.0 × 106 in Nicotiana tabacum plants systemically infected with tobacco mosaic virus (TMV). Cucumber mosaic virus (CMV)-infected Pachystachys coccinea plants contained 2 minor bands of MWs 0.49 and 0.35 × 106 in addition to the previously described 4 major ds RNAs and ds CARNA 5 (MW 0.22 × 106). The patterns of ds RNA are useful for diagnosing natural infections of CMV and TMV in N. glauca plants and of citrus tristeza virus in Citrus spp. © 1983.
Scientific Publication
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