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פותח על ידי קלירמאש פתרונות בע"מ -
An efficient in vitro clonal propagation method for Aconitum napellus
Year:
1997
Source of publication :
Acta Horticulturae
Authors :
גאבה, ויקטור
;
.
ותד, עבד
;
.
כוכבא, מרנינה
;
.
ניסים-לוי, עדה
;
.
Volume :
430
Co-Authors:
Kochba, M., Depts. of Ornamental Horticulture and Virology, Agricultural Research Organization, P.O.B. 6, Bet Dagan 50250, Israel
Gaba, V., Depts. of Ornamental Horticulture and Virology, Agricultural Research Organization, P.O.B. 6, Bet Dagan 50250, Israel
Nissim, A., Depts. of Ornamental Horticulture and Virology, Agricultural Research Organization, P.O.B. 6, Bet Dagan 50250, Israel
Watad, A.A., Depts. of Ornamental Horticulture and Virology, Agricultural Research Organization, P.O.B. 6, Bet Dagan 50250, Israel
Facilitators :
From page:
281
To page:
287
(
Total pages:
7
)
Abstract:
An efficient method was developed using floating membrane rafts (Liferaft™) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing GA3 - treated shoot tips to culture on benzyl adenine (BA) - supplemented solid Murashige and Skoog (1962) media or onto membrane rafts floating on liquid media of the same composition. Optimum shoot proliferation on solid medium required 4mgl-1 BA, whereas for explants supported on rafts optimal proliferation was achieved at 0.25mgl -1 BA. Maximum shoot proliferation was found using the floating rafts (propagation ratio of 4.2 per month), 45% higher than on solid medium. A similar value could be obtained on solid medium after a period of 2 months. The optimal response to BA was similar for fresh weight gain and shoot length. Growth in a shallow layer of liquid in shake flasks gave a similar shoot multiplication rate to that on floating rafts; however, submerged leaves browned and died. The above results indicate the efficiency of using membrane rafts floating on liquid medium for in vitro propagation of Aconitum napellus. Using this method, many clones were propagated and flowered after planting in the greenhouse.
Note:
Related Files :
Aconitum napellus
Ranunculaceae
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר מתוך כינוס
;
.
Language:
אנגלית
Editors' remarks:
ID:
21989
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:48
Scientific Publication
An efficient in vitro clonal propagation method for Aconitum napellus
430
Kochba, M., Depts. of Ornamental Horticulture and Virology, Agricultural Research Organization, P.O.B. 6, Bet Dagan 50250, Israel
Gaba, V., Depts. of Ornamental Horticulture and Virology, Agricultural Research Organization, P.O.B. 6, Bet Dagan 50250, Israel
Nissim, A., Depts. of Ornamental Horticulture and Virology, Agricultural Research Organization, P.O.B. 6, Bet Dagan 50250, Israel
Watad, A.A., Depts. of Ornamental Horticulture and Virology, Agricultural Research Organization, P.O.B. 6, Bet Dagan 50250, Israel
An efficient in vitro clonal propagation method for Aconitum napellus
An efficient method was developed using floating membrane rafts (Liferaft™) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing GA3 - treated shoot tips to culture on benzyl adenine (BA) - supplemented solid Murashige and Skoog (1962) media or onto membrane rafts floating on liquid media of the same composition. Optimum shoot proliferation on solid medium required 4mgl-1 BA, whereas for explants supported on rafts optimal proliferation was achieved at 0.25mgl -1 BA. Maximum shoot proliferation was found using the floating rafts (propagation ratio of 4.2 per month), 45% higher than on solid medium. A similar value could be obtained on solid medium after a period of 2 months. The optimal response to BA was similar for fresh weight gain and shoot length. Growth in a shallow layer of liquid in shake flasks gave a similar shoot multiplication rate to that on floating rafts; however, submerged leaves browned and died. The above results indicate the efficiency of using membrane rafts floating on liquid medium for in vitro propagation of Aconitum napellus. Using this method, many clones were propagated and flowered after planting in the greenhouse.
Scientific Publication
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