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פותח על ידי קלירמאש פתרונות בע"מ -
Suppression of fructokinase encoded by LeFRK2 in tomato stem inhibits growth and causes wilting of young leaves
Year:
2003
Source of publication :
Plant Journal
Authors :
ברנשטיין, נירית
;
.
גרמן, מרסלו
;
.
גרנות, דוד
;
.
דאי, ניר
;
.
חנאל, רנה
;
.
יופה, מרינה
;
.
מצביץ, טניה
;
.
פטרייקוב, מרינה
;
.
שחק, יוספה
;
.
שפר, ארתור
;
.
Volume :
34
Co-Authors:
German, M.A., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Dai, N., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Matsevitz, T., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Hanael, R., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Petreikov, M., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Bernstein, N., Inst. of Soils, Water/Environ. Sci., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Ioffe, M., Inst. of Soils, Water/Environ. Sci., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Shahak, Y., Institute of Horticulture, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Schaffer, A.A., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Granot, D., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
837
To page:
846
(
Total pages:
10
)
Abstract:
Fructokinases catalyze the key step of fructose phosphorylation in plants. LeFRK2, the major fructokinase-encoding gene in tomato plants, is abundantly expressed in roots, stems, and fruits. To analyze the role of LeFRK2 in plant development, we analyzed transgenic tomato plants with sense and antisense expression of StFRK, the potato homolog of LeFRK2. Increased fructokinase activity had no effect. However, plants in which LeFRK2 was specifically suppressed, either via antisense suppression or via co-suppression, exhibited growth inhibition and wilting of young leaves at daytime. Grafting experiments indicated that a stem interstock of antisense plants was sufficient to inhibit growth and cause leaf wilting. Stem secondary xylem exhibited particular suppression of LeFRK2 and the area of active xylem, estimated by eosin uptake, was significantly smaller in antisense stem compared to that of wild-type plants. These results suggest that LeFRK2 might be required for proper development of xylem that affected growth and wilting.
Note:
Related Files :
antisense
Co-suppression
Eukaryota
Fructokinases
fructose
Fruits
Hexose phosphorylation
Plant Leaves
Solanum tuberosum
עוד תגיות
תוכן קשור
More details
DOI :
10.1046/j.1365-313X.2003.01765.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22005
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:48
Scientific Publication
Suppression of fructokinase encoded by LeFRK2 in tomato stem inhibits growth and causes wilting of young leaves
34
German, M.A., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Dai, N., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Matsevitz, T., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Hanael, R., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Petreikov, M., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Bernstein, N., Inst. of Soils, Water/Environ. Sci., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Ioffe, M., Inst. of Soils, Water/Environ. Sci., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Shahak, Y., Institute of Horticulture, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Schaffer, A.A., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Granot, D., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Suppression of fructokinase encoded by LeFRK2 in tomato stem inhibits growth and causes wilting of young leaves
Fructokinases catalyze the key step of fructose phosphorylation in plants. LeFRK2, the major fructokinase-encoding gene in tomato plants, is abundantly expressed in roots, stems, and fruits. To analyze the role of LeFRK2 in plant development, we analyzed transgenic tomato plants with sense and antisense expression of StFRK, the potato homolog of LeFRK2. Increased fructokinase activity had no effect. However, plants in which LeFRK2 was specifically suppressed, either via antisense suppression or via co-suppression, exhibited growth inhibition and wilting of young leaves at daytime. Grafting experiments indicated that a stem interstock of antisense plants was sufficient to inhibit growth and cause leaf wilting. Stem secondary xylem exhibited particular suppression of LeFRK2 and the area of active xylem, estimated by eosin uptake, was significantly smaller in antisense stem compared to that of wild-type plants. These results suggest that LeFRK2 might be required for proper development of xylem that affected growth and wilting.
Scientific Publication
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