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פותח על ידי קלירמאש פתרונות בע"מ -
Expression of Active Human Interferon-β in Transgenic Plants
Year:
1992
Source of publication :
Journal of Interferon Research
Authors :
גפני, ידידיה
;
.
הולנד, נטע
;
.
שטיין, דנה
;
.
Volume :
12
Co-Authors:
Edelbaum, O., Virus Laboratory, Otto Warburg Center for Biotechnology, Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot 76100, Israel
Stein, D., Department of Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Holland, N., Department of Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Gafni, Y., Department of Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Livneh, O., Virus Laboratory, Otto Warburg Center for Biotechnology, Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot 76100, Israel
Novick, D., Department of Virology, Weizmann Institute of Science, Rehovot 76100, Israel
Rubinstein, M., Department of Virology, Weizmann Institute of Science, Rehovot 76100, Israel
Sela, I., Virus Laboratory, Otto Warburg Center for Biotechnology, Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot 76100, Israel
Facilitators :
From page:
449
To page:
453
(
Total pages:
5
)
Abstract:
Tobacco plants were transformed with the human gene for interferon-β (IFN-β). Transformation was determined by the polymerase chain reaction (PCR), and expression was determined by Western blot analysis, by purifying the IFN from the transgenic plants, and by bioassays indicating its activity in human cells. Plants expressing IFN-β were self-pollinated. IFN-β-expressing progeny plants were selected and produced active IFN-β, indicating stable transformation. © 1992, Mary Ann Liebert, Inc. All rights reserved.
Note:
Related Files :
antiviral activity
Base Sequence
gene expression
genetic transformation
interferon induction
Tobacco mosaic virus
עוד תגיות
תוכן קשור
More details
DOI :
10.1089/jir.1992.12.449
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22011
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:48
Scientific Publication
Expression of Active Human Interferon-β in Transgenic Plants
12
Edelbaum, O., Virus Laboratory, Otto Warburg Center for Biotechnology, Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot 76100, Israel
Stein, D., Department of Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Holland, N., Department of Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Gafni, Y., Department of Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Livneh, O., Virus Laboratory, Otto Warburg Center for Biotechnology, Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot 76100, Israel
Novick, D., Department of Virology, Weizmann Institute of Science, Rehovot 76100, Israel
Rubinstein, M., Department of Virology, Weizmann Institute of Science, Rehovot 76100, Israel
Sela, I., Virus Laboratory, Otto Warburg Center for Biotechnology, Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot 76100, Israel
Expression of Active Human Interferon-β in Transgenic Plants
Tobacco plants were transformed with the human gene for interferon-β (IFN-β). Transformation was determined by the polymerase chain reaction (PCR), and expression was determined by Western blot analysis, by purifying the IFN from the transgenic plants, and by bioassays indicating its activity in human cells. Plants expressing IFN-β were self-pollinated. IFN-β-expressing progeny plants were selected and produced active IFN-β, indicating stable transformation. © 1992, Mary Ann Liebert, Inc. All rights reserved.
Scientific Publication
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