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פותח על ידי קלירמאש פתרונות בע"מ -
An intracellular Laccase is responsible for epicatechin-mediated Anthocyanin degradation in litchi fruit Pericarp
Year:
2015
Authors :
אורן-שמיר, מיכל
;
.
Volume :
169
Co-Authors:
Fang, F., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China, Research Institute of Food Science and Engineering Technology, Hezhou University, Hezhou, China
Zhang, X.-L., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Luo, H.-H., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Zhou, J.-J., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Gong, Y.-H., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Horticulture, South China Agricultural University, Guangzhou, China
Li, W.-J., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Shi, Z.-W., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
He, Q., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Wu, Q., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Li, L., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Jiang, L.-L., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Cai, Z.-G., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Oren-Shamir, M., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Centre, Bet Dagan, Israel
Zhang, Z.-Q., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Horticulture, South China Agricultural University, Guangzhou, China
Pang, X.-Q., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Facilitators :
From page:
2391
To page:
2408
(
Total pages:
18
)
Abstract:
In contrast to the detailed molecular knowledge available on anthocyanin synthesis, little is known about its catabolism in plants. Litchi (Litchi chinensis) fruit lose their attractive red color soon after harvest. The mechanism leading to quick degradation of anthocyanins in the pericarp is not well understood. An anthocyanin degradation enzyme (ADE) was purified to homogeneity by sequential column chromatography, using partially purified anthocyanins from litchi pericarp as a substrate. The purified ADE, of 116 kD by urea SDS-PAGE, was identified as a laccase (ADE/LAC). The full-length complementary DNA encoding ADE/LAC was obtained, and a polyclonal antibody raised against a deduced peptide of the gene recognized the ADE protein. The anthocyanin degradation function of the gene was confirmed by its transient expression in tobacco (Nicotiana benthamiana) leaves. The highest ADE/LAC transcript abundance was in the pericarp in comparison with other tissues, and was about 1,000-fold higher than the polyphenol oxidase gene in the pericarp. Epicatechin was found to be the favorable substrate for the ADE/LAC. The dependence of anthocyanin degradation by the enzyme on the presence of epicatechin suggests an ADE/LAC epicatechin-coupled oxidation model. This model was supported by a dramatic decrease in epicatechin content in the pericarp parallel to anthocyanin degradation. Immunogold labeling transmission electron microscopy suggested that ADE/LAC is located mainly in the vacuole, with essential phenolic substances. ADE/LAC vacuolar localization, high expression levels in the pericarp, and high epicatechindependent anthocyanin degradation support its central role in pigment breakdown during pericarp browning. © 2015 American Society of Plant Biologists. All rights reserved.
Note:
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More details
DOI :
10.1104/pp.15.00359
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22012
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:48
Scientific Publication
An intracellular Laccase is responsible for epicatechin-mediated Anthocyanin degradation in litchi fruit Pericarp
169
Fang, F., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China, Research Institute of Food Science and Engineering Technology, Hezhou University, Hezhou, China
Zhang, X.-L., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Luo, H.-H., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Zhou, J.-J., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Gong, Y.-H., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Horticulture, South China Agricultural University, Guangzhou, China
Li, W.-J., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Shi, Z.-W., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
He, Q., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Wu, Q., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Li, L., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Jiang, L.-L., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Cai, Z.-G., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
Oren-Shamir, M., Department of Ornamental Horticulture, Agriculture Research Organization, Volcani Centre, Bet Dagan, Israel
Zhang, Z.-Q., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Horticulture, South China Agricultural University, Guangzhou, China
Pang, X.-Q., State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China, College of Life Sciences, South China Agricultural University, Guangzhou, China
An intracellular Laccase is responsible for epicatechin-mediated Anthocyanin degradation in litchi fruit Pericarp
In contrast to the detailed molecular knowledge available on anthocyanin synthesis, little is known about its catabolism in plants. Litchi (Litchi chinensis) fruit lose their attractive red color soon after harvest. The mechanism leading to quick degradation of anthocyanins in the pericarp is not well understood. An anthocyanin degradation enzyme (ADE) was purified to homogeneity by sequential column chromatography, using partially purified anthocyanins from litchi pericarp as a substrate. The purified ADE, of 116 kD by urea SDS-PAGE, was identified as a laccase (ADE/LAC). The full-length complementary DNA encoding ADE/LAC was obtained, and a polyclonal antibody raised against a deduced peptide of the gene recognized the ADE protein. The anthocyanin degradation function of the gene was confirmed by its transient expression in tobacco (Nicotiana benthamiana) leaves. The highest ADE/LAC transcript abundance was in the pericarp in comparison with other tissues, and was about 1,000-fold higher than the polyphenol oxidase gene in the pericarp. Epicatechin was found to be the favorable substrate for the ADE/LAC. The dependence of anthocyanin degradation by the enzyme on the presence of epicatechin suggests an ADE/LAC epicatechin-coupled oxidation model. This model was supported by a dramatic decrease in epicatechin content in the pericarp parallel to anthocyanin degradation. Immunogold labeling transmission electron microscopy suggested that ADE/LAC is located mainly in the vacuole, with essential phenolic substances. ADE/LAC vacuolar localization, high expression levels in the pericarp, and high epicatechindependent anthocyanin degradation support its central role in pigment breakdown during pericarp browning. © 2015 American Society of Plant Biologists. All rights reserved.
Scientific Publication
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