Co-Authors:
Raikhinstein, M., Department of Hormone Research, Weizmann Institute of Science, Rehovot, 76100, Israel
Hanukoglu, I., Department of Hormone Research, Weizmann Institute of Science, Rehovot, 76100, Israel
Abstract:
A rapid procedure for the isolation of λ phage DNA is described. The method combines the advantages of direct lysate ultracentrifugation with phage purification on a DEAE-cellulose minicolumn. It can be applied to either liquid or plate lysates on a small or medium scale. The isolated DNA is suitable for restriction and direct sequencing.