אסיף מאגר המחקר החקלאי

Synthesis and biological activity of a photoaffinity-biotinylated pheromone-biosynthesis activating neuropeptide (PBAN) analog

Year:

1999

Source of publication :

Authors :

Volume :

20

Co-Authors:

Rafaeli, A., Department of Stored Products, Pheromone Res. Lab., Volcani C., Bet Dagan, Israel
Gileadi, C., Department of Stored Products, Pheromone Res. Lab., Volcani C., Bet Dagan, Israel

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Abstract:

To study the mode of action of pheromone-biosynthesis activating neuropeptide (PBAN) at the receptor level and for receptor purification, we synthesized and tested the biologic properties of a photoaffinity biotinylated PBAN analog N-[N-(4-azido-tetrafluorobenzoyl)-biocytinyloxyl-succinimide (Atf-Bct-NHS-PBAN). The Atf-Bct-NHS-PBAN was separated from unreacted reagent and synthetic Hez-PBAN by high-performance liquid chromatography. Conjugated biotin was detected by using enzyme-linked assay as well as tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis. The biologic activity of purified Atf-Bct-NHS-PBAN was confirmed using both in vivo and in vitro pheromonotropic bioassays. These observations indicate that Atf-Bct-NHS-PBAN is a full agonist of PBAN action in pheromone glands and may be used to study PBAN receptors by employing avidin coupled to various reporter groups. Copyright (C) 1999 Elsevier Science Inc.

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