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פותח על ידי קלירמאש פתרונות בע"מ -
Degradation of 2,4,6-tribromophenol by bacterial cells attached to chalk collected from a contaminated aquifer
Year:
2004
Source of publication :
Journal of Applied Microbiology
Authors :
סעדי, אברהים
;
.
Volume :
96
Co-Authors:
Aim: To investigate the factors governing the adhesion and activity of the 2,4,6-tribromophenol (TBP) degrading bacterium Achromobacter piechaudii TBPZ-N61 on chalk from a contaminated aquifer. Methods and Results: Adhesion kinetics of TBPZ-N61 to grey and white chalk from a polluted fractured chalk aquifer was tested in a batch system. Both grey and white chalk contain ca 80% CaCO3, while grey chalk contains more organic matter (2.4%) than the white chalk (0.3%) and also contains Dolmite and Clinoptilolite. Adhesion of the bacterial cells to the chalk particles (<
0.2 mm) occurred rapidly (96% of the cells within 15 min). Langmuirfitted adhesion isotherms suggest that cells in the stationary phase, which are more hydrophobic, adhere to both grey and white chalk more efficiently than cells in the logarithmic growth phase. Increasing the pH (from 6.7 to 8.1) caused a significant reduction in cell adhesion to the chalk. Activity of attached cells was evaluated in both batch and column experiments. Logarithmic cells adhering to white and grey chalk were more active in TBP degradation than cells in suspension. In column experiments, significant TBP degradation was retained up to 30 days after a single injection of TBPZ cells. Thereafter, activity was fully recovered by amendment of yeast extract. Chalk surfaces that were incubated in situ in contaminated groundwater for 20 days still allowed the adhesion and activity of TBPZ cells. Conclusions: Taken together, our results show that bacteria adhere efficiently to specific sites on the chalk surfaces, and that sustained bacterial activity of the attached cells can be achieved by adding a carbon source such as yeast extract which also overcome toxic constituents that may occur in some chalk types. Significance and Impact of the Study: Bioremediation of TBP-contaminated chalk aquifers is made possible by the injection of bacterial cultures.
Facilitators :
From page:
844
To page:
852
(
Total pages:
9
)
Abstract:
Achromobacter; Adhesion; Fractured chalk; Hydrophobicity; Subsurface; Tribromophenol degradation
Note:
Related Files :
achromobacter piechaudii
Biodegradation
Environmental Microbiology
Ground Water
Microbial degradation
pH
phenols
unclassified drug
עוד תגיות
תוכן קשור
More details
DOI :
https://www.scopus.com/inward/record.uri?eid=2-s2.0-1842505971&doi=10.1111%2fj.1365-2672.2004.02222.x&partnerID=40&md5=67a0ea67930bc1addfa7160646c93318
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22038
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:48
Scientific Publication
Degradation of 2,4,6-tribromophenol by bacterial cells attached to chalk collected from a contaminated aquifer
96
Aim: To investigate the factors governing the adhesion and activity of the 2,4,6-tribromophenol (TBP) degrading bacterium Achromobacter piechaudii TBPZ-N61 on chalk from a contaminated aquifer. Methods and Results: Adhesion kinetics of TBPZ-N61 to grey and white chalk from a polluted fractured chalk aquifer was tested in a batch system. Both grey and white chalk contain ca 80% CaCO3, while grey chalk contains more organic matter (2.4%) than the white chalk (0.3%) and also contains Dolmite and Clinoptilolite. Adhesion of the bacterial cells to the chalk particles (<
0.2 mm) occurred rapidly (96% of the cells within 15 min). Langmuirfitted adhesion isotherms suggest that cells in the stationary phase, which are more hydrophobic, adhere to both grey and white chalk more efficiently than cells in the logarithmic growth phase. Increasing the pH (from 6.7 to 8.1) caused a significant reduction in cell adhesion to the chalk. Activity of attached cells was evaluated in both batch and column experiments. Logarithmic cells adhering to white and grey chalk were more active in TBP degradation than cells in suspension. In column experiments, significant TBP degradation was retained up to 30 days after a single injection of TBPZ cells. Thereafter, activity was fully recovered by amendment of yeast extract. Chalk surfaces that were incubated in situ in contaminated groundwater for 20 days still allowed the adhesion and activity of TBPZ cells. Conclusions: Taken together, our results show that bacteria adhere efficiently to specific sites on the chalk surfaces, and that sustained bacterial activity of the attached cells can be achieved by adding a carbon source such as yeast extract which also overcome toxic constituents that may occur in some chalk types. Significance and Impact of the Study: Bioremediation of TBP-contaminated chalk aquifers is made possible by the injection of bacterial cultures.
Degradation of 2,4,6-tribromophenol by bacterial cells attached to chalk collected from a contaminated aquifer
Achromobacter; Adhesion; Fractured chalk; Hydrophobicity; Subsurface; Tribromophenol degradation
Scientific Publication
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