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פותח על ידי קלירמאש פתרונות בע"מ -
Screening common bean (Phaseolus vulgaris) for resistance to Tomato yellow leaf curl virus
Year:
2002
Source of publication :
Plant Disease
Authors :
לפידות, משה
;
.
Volume :
86
Co-Authors:
Lapidot, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
429
To page:
432
(
Total pages:
4
)
Abstract:
Commercial cultivars of common bean (Phaseolus vulgaris) were screened for resistance to Tomato yellow leaf curl virus (TYLCV). Fourteen-day-old bean plants were inoculated with TYLCV by means of the whitefly vector (Bemisia tabaci). Following inoculation, plants were sprayed with an insecticide, kept in an insect-proof greenhouse for 4 weeks, and monitored for disease symptom development. The presence of viral DNA in the inoculated plants was assayed by dot blot hybridization and by polymerase chain reaction. Out of the 42 cultivars that were tested, 24 were found to be susceptible: the plants exhibited severe symptoms and accumulated high levels of viral DNA. In all, 18 cultivars were found to be resistant to the virus: 1 cultivar showed mild symptoms while the other 17 showed no symptoms following inoculation. From the 17 symptomless cultivars, plants of 3 cultivars contained viral DNA while no viral DNA was detected in the plants of the other 14 cultivars. When the effect of bean plant age on viral inoculation by whiteflies was assayed, it was found that the success rate of TYLCV infection was highly dependent on bean plant age. When 14-day-old susceptible bean plants were inoculated, nearly 100% infection was achieved but, when 26- or 12-day-old plants of the same cultivar were inoculated, the infection rates were only 34 and 40%, respectively.
Note:
Related Files :
Bemisia tabaci
DNA
enzymes
greenhouses
insecticides
Ipomoea batatas
Solanum tuberosum
Tomato yellow leaf curl virus
Viruses
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22046
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:48
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Scientific Publication
Screening common bean (Phaseolus vulgaris) for resistance to Tomato yellow leaf curl virus
86
Lapidot, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Screening common bean (Phaseolus vulgaris) for resistance to Tomato yellow leaf curl virus
Commercial cultivars of common bean (Phaseolus vulgaris) were screened for resistance to Tomato yellow leaf curl virus (TYLCV). Fourteen-day-old bean plants were inoculated with TYLCV by means of the whitefly vector (Bemisia tabaci). Following inoculation, plants were sprayed with an insecticide, kept in an insect-proof greenhouse for 4 weeks, and monitored for disease symptom development. The presence of viral DNA in the inoculated plants was assayed by dot blot hybridization and by polymerase chain reaction. Out of the 42 cultivars that were tested, 24 were found to be susceptible: the plants exhibited severe symptoms and accumulated high levels of viral DNA. In all, 18 cultivars were found to be resistant to the virus: 1 cultivar showed mild symptoms while the other 17 showed no symptoms following inoculation. From the 17 symptomless cultivars, plants of 3 cultivars contained viral DNA while no viral DNA was detected in the plants of the other 14 cultivars. When the effect of bean plant age on viral inoculation by whiteflies was assayed, it was found that the success rate of TYLCV infection was highly dependent on bean plant age. When 14-day-old susceptible bean plants were inoculated, nearly 100% infection was achieved but, when 26- or 12-day-old plants of the same cultivar were inoculated, the infection rates were only 34 and 40%, respectively.
Scientific Publication
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