Microcutting culture and morpho-physiological changes during acclimation in two Lycium chilense cytotypes

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הספר "אוצר וולקני"

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תנאי שימוש

פותח על ידי קלירמאש פתרונות בע"מ -

Microcutting culture and morpho-physiological changes during acclimation in two Lycium chilense cytotypes

Year: 

2004

Source of publication :

Biocell

Authors :

למקוף, חורחה הוגו

.

Volume :

Co-Authors: 

Maseda, P.H., IFEVA - Cat. de Fisiologia Vegetal, CONICET-FAUBA, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina, Catedra de Fisiologia Vegetal, Facultad de Agronomia, Universidad de Buenos Aires, Avda. San Martín 4453, (C1417DSE) Buenos Aires, Argentina
Lemcoff, J.H., IFEVA - Cat. de Fisiologia Vegetal, CONICET-FAUBA, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina
Murúa, M., Department of Agronomy, Purdue University, Lilly Hall 1150, West Lafayette, IN 47907-1150, United States
Frayssinet, N., Catedra de Genetica (FAUBA), Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE Buenos Aires, Argentina
Carceller, M.S., IFEVA - Cat. de Fisiologia Vegetal, CONICET-FAUBA, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina

From page: 

271

To page: 

277

(

Total pages: 

7

)

Link :

Microcutting culture and morpho-physiological changes during acclimation in two Lycium chilense cytotypes

Abstract: 

Lycium chilense, a deciduous perennial shrub, is one of the endangered native species of Patagonia due to sheep overgrazing. Chances of recolonization by seeds are scarce due to the limited density of propagules in the soil and very specific requirements for germination. The objective was to develop an in vitro propagation protocol that would help to perform reestablishment of this species in degraded areas of the Patagonian steppe. Seeds came from two provenances with different somatic number due to differences in ploidy level. Defoliated microcuttings were planted in test tubes with different growing media and taken to a growth chamber. Rooting percentage did not differ between origins, but higher values were encountered for medium without hormones. Subcultures increased significantly rooting percentage and reduced time to rooting. The leaves from micropropagated plants were thinner, did not exhibit hairs, and had poorly developed palisade parenchyma and less epicuticular waxes. In vitro leaves had lower stomatal density and their stomata were less functional when compared to acclimated leaves. A repopulation program of Lycium chilense based on microcutting culture, specially using subcultures, is feasible.