אסיף מאגר המחקר החקלאי

'Retroposon' insertion into the cellular oncogene c-myc in canine transmissible venereal tumor

Year:

1985

Source of publication :

Authors :

Volume :

82

Co-Authors:

Katzir, N., Department of Chemical Immunology, The Weizmann Institute, Rehovoth, Israel
Rechavi, G., Department of Chemical Immunology, The Weizmann Institute, Rehovoth, Israel
Cohen, J.B., Department of Chemical Immunology, The Weizmann Institute, Rehovoth, Israel
Unger, T.
Simoni, F.
Segal, S.
Givol, D.

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Abstract:

We examined by Southern blotting the state of the cellular oncogene c-myc in the dog transmissible venereal tumor. The tumor DNA contains a 16.8-kilobase pair (kbp) rearranged c-myc fragment in addition to the normal 15-kbp and 7.5-kbp fragments. We compared the structure of the cloned rearranged c-myc (rc-myc) with that of a cloned normal c-myc and found that the rearrangement was due to the insertion of a 1.8-kbp DNA upstream to the first exon of c-myc. The inserted DNA is flanked by 10-base-pair direct repeats and contains a dA-rich tail, suggesting its origin from mRNA. Partial sequence of the inserted element showed 62% homology with the primate interdispersed Kpn I repetitive element. These results provide an example for the behavior of repetitive DNA sequences like the Kpn I family, as movable elements that can transpose nearby to oncogenes or other structural genes and perhaps affect their activity.

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