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פותח על ידי קלירמאש פתרונות בע"מ -
Improved medium for selecting nitrate-nonutilizing (nit) mutants of Verticillium dahliae
Year:
1997
Source of publication :
Phytopathology
Authors :
קורולב, נדיה
;
.
קטן, תלמה
;
.
Volume :
87
Co-Authors:

Korolev, N., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Katan, T., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel

Facilitators :
From page:
1067
To page:
1070
(
Total pages:
4
)
Abstract:
Nitrate-nonutilizing (nit) mutants are commonly used to determine vegetative compatibility between isolates of Verticillium dahliae by complementation (heterokaryon) testing. These mutants emerge spontaneously as chlorate-resistant sectors growing out of partially restricted, wild-type colonies on chlorate-amended media. The commonly used chlorate media are based on minimal medium (MMC) or cornmeal agar (CMC), amended with potassium chlorate. nit mutants recovered on these media constituted 10 to 36% (on MMC) and 25 to 45% (on CMC) of the apparently resistant sectors. An improved water agar chlorate medium (WAC) is described that is more effective for selecting chlorate-resistant nit mutants. WAC medium consists of agar (2%), glucose (0.02%), and potassium chlorate (2 to 5%). On WAC, growth of most V. dahliae isolates was strongly inhibited, and 66 to 100% (average >80%) of the chlorate-resistant sectors formed were nit mutants. Most mutants were characterized as nit1, and about 6% as NitM.
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DOI :
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22134
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:49
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Scientific Publication
Improved medium for selecting nitrate-nonutilizing (nit) mutants of Verticillium dahliae
87

Korolev, N., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Katan, T., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel

Improved medium for selecting nitrate-nonutilizing (nit) mutants of Verticillium dahliae
Nitrate-nonutilizing (nit) mutants are commonly used to determine vegetative compatibility between isolates of Verticillium dahliae by complementation (heterokaryon) testing. These mutants emerge spontaneously as chlorate-resistant sectors growing out of partially restricted, wild-type colonies on chlorate-amended media. The commonly used chlorate media are based on minimal medium (MMC) or cornmeal agar (CMC), amended with potassium chlorate. nit mutants recovered on these media constituted 10 to 36% (on MMC) and 25 to 45% (on CMC) of the apparently resistant sectors. An improved water agar chlorate medium (WAC) is described that is more effective for selecting chlorate-resistant nit mutants. WAC medium consists of agar (2%), glucose (0.02%), and potassium chlorate (2 to 5%). On WAC, growth of most V. dahliae isolates was strongly inhibited, and 66 to 100% (average >80%) of the chlorate-resistant sectors formed were nit mutants. Most mutants were characterized as nit1, and about 6% as NitM.
Scientific Publication
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