נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
DNA barcoding of fish species from the Mediterranean coast of Israel
Year:
2016
Source of publication :
Mediterranean Marine Science
Authors :
דור, ליאור
;
.
חולתא, גדעון
;
.
סרוסי, אייל
;
.
רון, מיכה
;
.
שיראק, אנדרי
;
.
Volume :
17
Co-Authors:
Shirak, A., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Dor, L., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel, Department of Evolution, Systematics and Ecology, The Hebrew University of Jerusalem, Jerusalem, Israel
Seroussi, E., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Ron, M., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Hulata, G., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Golani, D., Department of Evolution, Systematics and Ecology, The Hebrew University of Jerusalem, Jerusalem, Israel
Facilitators :
From page:
459
To page:
466
(
Total pages:
8
)
Abstract:
Accurately classified genomic data in the Barcode of Life Data System (BOLD) database is vital to protecting and conserving marine biodiversity in the Mediterranean Sea. The taxonomic classifications of 468 fish of 50 Mediterranean species were analyzed using the BOLD Identifier tool for variation in the mitochondrial gene encoding cytochrome oxidase subunit I (COI). Within species, nucleotide maximum composite likelihood was low with a mean of 0.0044 ± 0.0008. Three presumptive species had significantly higher values: Arnoglossus spp. (0.07), Torquigener flavimaculosus (0.013) and Boops boops (0.028). However, the Arnoglossus spp. samples were subclassified into two groups that were ultimately identified as two different species: Arnoglossus laterna and Arnoglossus thori. For the different species, BLAST searches against the BOLD database using our DNA barcoding data as the query sequences categorized the most similar targets into groups. For each analyzed species, the similarity of the first and second threshold groups ranged from 95 to 99% and from 83 to 98%, respectively. Sequence-based classification for the first threshold group was concordant with morphology-based identification. However, for 34 analyzed species (68%), overlaps of species between the two threshold groups hampered classification. Tree-based phylogenetic analysis detected more than one cluster in the first threshold group for 22 out of 50 species, representing genetic subgroups and geographic origins. There was a tendency for higher conservation and a lower number of clusters in the Lessepsian (Red Sea) migrant versus indigenous species.
Note:
Related Files :
COI barcoding
Lessepsian migration
Sequence-based classification
עוד תגיות
תוכן קשור
More details
DOI :
10.12681/mms.1384
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22202
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:50
Scientific Publication
DNA barcoding of fish species from the Mediterranean coast of Israel
17
Shirak, A., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Dor, L., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel, Department of Evolution, Systematics and Ecology, The Hebrew University of Jerusalem, Jerusalem, Israel
Seroussi, E., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Ron, M., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Hulata, G., Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Golani, D., Department of Evolution, Systematics and Ecology, The Hebrew University of Jerusalem, Jerusalem, Israel
DNA barcoding of fish species from the Mediterranean coast of Israel
Accurately classified genomic data in the Barcode of Life Data System (BOLD) database is vital to protecting and conserving marine biodiversity in the Mediterranean Sea. The taxonomic classifications of 468 fish of 50 Mediterranean species were analyzed using the BOLD Identifier tool for variation in the mitochondrial gene encoding cytochrome oxidase subunit I (COI). Within species, nucleotide maximum composite likelihood was low with a mean of 0.0044 ± 0.0008. Three presumptive species had significantly higher values: Arnoglossus spp. (0.07), Torquigener flavimaculosus (0.013) and Boops boops (0.028). However, the Arnoglossus spp. samples were subclassified into two groups that were ultimately identified as two different species: Arnoglossus laterna and Arnoglossus thori. For the different species, BLAST searches against the BOLD database using our DNA barcoding data as the query sequences categorized the most similar targets into groups. For each analyzed species, the similarity of the first and second threshold groups ranged from 95 to 99% and from 83 to 98%, respectively. Sequence-based classification for the first threshold group was concordant with morphology-based identification. However, for 34 analyzed species (68%), overlaps of species between the two threshold groups hampered classification. Tree-based phylogenetic analysis detected more than one cluster in the first threshold group for 22 out of 50 species, representing genetic subgroups and geographic origins. There was a tendency for higher conservation and a lower number of clusters in the Lessepsian (Red Sea) migrant versus indigenous species.
Scientific Publication
You may also be interested in