נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Intestinal B0AT1 (SLC6A19) and PEPT1 (SLC15A1) mRNA levels in European sea bass (Dicentrarchus labrax) reared in fresh water and fed fish and plant protein sources
Year:
2015
Source of publication :
Journal of Nutritional Science
Authors :
הרפז, שנאן
;
.
Volume :
4
Co-Authors:
Rimoldi, S., Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy
Bossi, E., Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy
Harpaz, S., Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Cattaneo, A.G., Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy
Bernardini, G., Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy, Inter-University Centre for Research in Protein Biotechnologies, Protein Factory, Polytechnic University of Milan, University of Insubria, Varese, Italy
Saroglia, M., Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy, Inter-University Centre for Research in Protein Biotechnologies, Protein Factory, Polytechnic University of Milan, University of Insubria, Varese, Italy
Terova, G., Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy, Inter-University Centre for Research in Protein Biotechnologies, Protein Factory, Polytechnic University of Milan, University of Insubria, Varese, Italy
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
The objective of the present study was to examine the effect of diets with descending fish meal (FM) inclusion levels and the addition of salt to the diet containing the lowest FM level on growth performances, feed conversion ratio, and intestinal solute carrier family 6 member 19 (SLC6A19) and oligopeptide transporter 1 (PEPT1) transcript levels, in freshwater-adapted European sea bass (Dicentrarchus labrax). We first isolated by molecular cloning and sequenced a full-length cDNA representing the neutral amino acid transporter SLC6A19 in sea bass. The cDNA sequence was deposited in GenBank database (accession no. KC812315). The twelve transmembrane domains and the 'de novo' prediction of the three-dimensional structure of SLC6A19 protein (634 amino acids) are presented. We then analysed diet-induced changes in the mRNA copies of SLC6A19 and PEPT1 genes in different portions of sea bass intestine using real-time RT-PCR. Sea bass were fed for 6 weeks on different diets, with ascending levels of fat or descending levels of FM, which was replaced with vegetable meal. The salt-enriched diet was prepared by adding 3 % NaCl to the diet containing 10 % FM. SLC6A19 mRNA in the anterior and posterior intestine of sea bass were not modulated by dietary protein sources and salt supplementation. Conversely, including salt in a diet containing a low FM percentage up-regulated the mRNA copies of PEPT1 in the hindgut. Fish growth correlated positively with the content of FM in the diets. Interestingly, the addition of salt to the diet containing 10 % FM improved feed intake, as well as specific growth rate and feed conversion ratio. © The Author(s) 2015.
Note:
Related Files :
aquaculture1 (domain1)
Dietary salt addition
Fish meal replacement
gene expression
Real-time PCR
Vegetable meal
עוד תגיות
תוכן קשור
More details
DOI :
10.1017/jns.2015.9
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22209
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:50
You may also be interested in
Scientific Publication
Intestinal B0AT1 (SLC6A19) and PEPT1 (SLC15A1) mRNA levels in European sea bass (Dicentrarchus labrax) reared in fresh water and fed fish and plant protein sources
4
Rimoldi, S., Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy
Bossi, E., Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy
Harpaz, S., Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Cattaneo, A.G., Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy
Bernardini, G., Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy, Inter-University Centre for Research in Protein Biotechnologies, Protein Factory, Polytechnic University of Milan, University of Insubria, Varese, Italy
Saroglia, M., Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy, Inter-University Centre for Research in Protein Biotechnologies, Protein Factory, Polytechnic University of Milan, University of Insubria, Varese, Italy
Terova, G., Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy, Inter-University Centre for Research in Protein Biotechnologies, Protein Factory, Polytechnic University of Milan, University of Insubria, Varese, Italy
Intestinal B0AT1 (SLC6A19) and PEPT1 (SLC15A1) mRNA levels in European sea bass (Dicentrarchus labrax) reared in fresh water and fed fish and plant protein sources
The objective of the present study was to examine the effect of diets with descending fish meal (FM) inclusion levels and the addition of salt to the diet containing the lowest FM level on growth performances, feed conversion ratio, and intestinal solute carrier family 6 member 19 (SLC6A19) and oligopeptide transporter 1 (PEPT1) transcript levels, in freshwater-adapted European sea bass (Dicentrarchus labrax). We first isolated by molecular cloning and sequenced a full-length cDNA representing the neutral amino acid transporter SLC6A19 in sea bass. The cDNA sequence was deposited in GenBank database (accession no. KC812315). The twelve transmembrane domains and the 'de novo' prediction of the three-dimensional structure of SLC6A19 protein (634 amino acids) are presented. We then analysed diet-induced changes in the mRNA copies of SLC6A19 and PEPT1 genes in different portions of sea bass intestine using real-time RT-PCR. Sea bass were fed for 6 weeks on different diets, with ascending levels of fat or descending levels of FM, which was replaced with vegetable meal. The salt-enriched diet was prepared by adding 3 % NaCl to the diet containing 10 % FM. SLC6A19 mRNA in the anterior and posterior intestine of sea bass were not modulated by dietary protein sources and salt supplementation. Conversely, including salt in a diet containing a low FM percentage up-regulated the mRNA copies of PEPT1 in the hindgut. Fish growth correlated positively with the content of FM in the diets. Interestingly, the addition of salt to the diet containing 10 % FM improved feed intake, as well as specific growth rate and feed conversion ratio. © The Author(s) 2015.
Scientific Publication
You may also be interested in