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פותח על ידי קלירמאש פתרונות בע"מ -
Differential expression pattern of inhibin α and βA subunits in the ovaries of postnatal and prepubertal lambs
Year:
1997
Authors :
בראב-טל, רות
;
.
זנו, אברהם
;
.
Volume :
9
Co-Authors:
Braw-Tal, R., Agricultural Research Organization, Volcani Center, Institute of Animal Science, Bet Dagan 50 250, Israel
Yossefi, S., Agricultural Research Organization, Volcani Center, Institute of Animal Science, Bet Dagan 50 250, Israel
Zenou, A., Agricultural Research Organization, Volcani Center, Institute of Animal Science, Bet Dagan 50 250, Israel
Bor, A., Agricultural Research Organization, Volcani Center, Institute of Animal Science, Bet Dagan 50 250, Israel
Facilitators :
From page:
825
To page:
832
(
Total pages:
8
)
Abstract:
The aim of the present study was to characterize gene and protein expression of follistatin, inhibin α (α) and inhibin βA (βA) subunits in the ovaries of postnatal (3-week-old) and prepubertal (14 and 20-25-week- old) lambs. Northern blot analysis revealed the presence of two α and two βA mRNAs. In postnatal ovary the α 1.2-kb transcript was abundant, its amount gradually falling, while the 2.0-kb mRNA increased and became a major band at 20-25 weeks. Both βA mRNAs, 4.5 kb and 6.0-7.5 kb, were weakly expressed in postnatal ovary, but whereas the 4.5-kb mRNA expression remained at a low level, that of the 6.0-7.5 kb mRNA increased about five fold in prepubertal ovary. The ratio of total α mRNAs to the dominant βA form (6.0- 7.5 kb) varied from 1.27 at 3 weeks to 0.33 at 20-25 weeks of age. One major follistatin mRNA of 2.5-3.6 kb was recognized and was constitutively expressed during ovarian growth. Several molecular-mass forms of α and βA subunits with different compositions were seen in prepubertal compared with postnatal ovaries, the latter exhibiting more active follicular growth. In summary, ovine ovaries undergo distinct changes early in life, both morphological and functional, and show a changing pattern of inhibin subunit expression.
Note:
Related Files :
animal experiment
Animals
Female
Growth Substances
ovariectomy
peptides
sheep
עוד תגיות
תוכן קשור
More details
DOI :
10.1071/R97073
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22247
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:50
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Scientific Publication
Differential expression pattern of inhibin α and βA subunits in the ovaries of postnatal and prepubertal lambs
9
Braw-Tal, R., Agricultural Research Organization, Volcani Center, Institute of Animal Science, Bet Dagan 50 250, Israel
Yossefi, S., Agricultural Research Organization, Volcani Center, Institute of Animal Science, Bet Dagan 50 250, Israel
Zenou, A., Agricultural Research Organization, Volcani Center, Institute of Animal Science, Bet Dagan 50 250, Israel
Bor, A., Agricultural Research Organization, Volcani Center, Institute of Animal Science, Bet Dagan 50 250, Israel
Differential expression pattern of inhibin α and βA subunits in the ovaries of postnatal and prepubertal lambs
The aim of the present study was to characterize gene and protein expression of follistatin, inhibin α (α) and inhibin βA (βA) subunits in the ovaries of postnatal (3-week-old) and prepubertal (14 and 20-25-week- old) lambs. Northern blot analysis revealed the presence of two α and two βA mRNAs. In postnatal ovary the α 1.2-kb transcript was abundant, its amount gradually falling, while the 2.0-kb mRNA increased and became a major band at 20-25 weeks. Both βA mRNAs, 4.5 kb and 6.0-7.5 kb, were weakly expressed in postnatal ovary, but whereas the 4.5-kb mRNA expression remained at a low level, that of the 6.0-7.5 kb mRNA increased about five fold in prepubertal ovary. The ratio of total α mRNAs to the dominant βA form (6.0- 7.5 kb) varied from 1.27 at 3 weeks to 0.33 at 20-25 weeks of age. One major follistatin mRNA of 2.5-3.6 kb was recognized and was constitutively expressed during ovarian growth. Several molecular-mass forms of α and βA subunits with different compositions were seen in prepubertal compared with postnatal ovaries, the latter exhibiting more active follicular growth. In summary, ovine ovaries undergo distinct changes early in life, both morphological and functional, and show a changing pattern of inhibin subunit expression.
Scientific Publication
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