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פותח על ידי קלירמאש פתרונות בע"מ -
Mn2+ alters peroxidase profiles and lignin degradation by the white-rot fungus Pleurotus ostreatus under different nutritional and growth conditions
Year:
2002
Authors :
כהן, רוני
;
.
Volume :
102-103
Co-Authors:
Cohen, R., Department of Plant Pathology, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Persky, L., Department of Plant Pathology, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Hazan-Eitan, Z., Department of Plant Pathology, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Yarden, O., Department of Plant Pathology, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Hadar, Y., Department of Plant Pathology, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Facilitators :
From page:
415
To page:
429
(
Total pages:
15
)
Abstract:
The white-rot fungus Pleurotus ostreatus produces two types of extracellular peroxidases: manganese-dependent peroxidase (MnP) and versatile peroxidase (VP). The effect of Mn2+ on fungal growth, peroxidase activity profiles, and lignin degradation by P. ostreatus was studied in liquid culture and under solid-state fermentation conditions on perlite, the latter resembling the natural growth conditions of this fungus. The fungus was grown in either a defined asparagine-containing basidiomycete selective medium (BSM) or in a rich peptone medium (PM). Biomass production, as determined by respiration experiments in solid-state fermentation and liquid cultures and fungal growth on Petri dishes, was higher in the PM than in the BSM. Mn2+ affected biomass production only in the PM on Petri dishes. In the nonamended PM, high levels of MnP and VP activity were detected relative to the nonamended BSM. Nevertheless, a higher rate of 14C-lignin mineralization was measured in the Mn2+-amended BSM, as determined during the course of 47 d of fermentation. Mn2+ amendment of the PM increased mineralization rate to that obtained in the Mn2+-amended BSM. The enzyme activity profiles of MnP and VP were studied in the BSM using anion-exchange chromatography. In the nonamended BSM, only minute levels of MnP and VP were detected. On Mn2+ amendment, two MnP isoenzymes (B1 and B2) appeared. Isoenzyme B2 was purified and showed 100% identity with the MnP isoenzyme purified in our previous study from PM-solid-state fermentation (P6). P6 was found to be the dominant isoenzyme in terms of activity level and gene expression compared with the VP isoenzymes. Based on these results, we concluded that Mn2+ plays a key role in lignin degradation under different nutritional and growth conditions, since it is required for the production of MnP in P. ostreatus.
Note:
Related Files :
anion exchange chromatography
Biomass
Carbon
Conference paper
fungi
gene expression
metabolism
Peptones
עוד תגיות
תוכן קשור
More details
DOI :
10.1385/ABAB:102-103:1-6:415
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר מתוך כינוס
;
.
Language:
אנגלית
Editors' remarks:
ID:
22258
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:50
You may also be interested in
Scientific Publication
Mn2+ alters peroxidase profiles and lignin degradation by the white-rot fungus Pleurotus ostreatus under different nutritional and growth conditions
102-103
Cohen, R., Department of Plant Pathology, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Persky, L., Department of Plant Pathology, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Hazan-Eitan, Z., Department of Plant Pathology, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Yarden, O., Department of Plant Pathology, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Hadar, Y., Department of Plant Pathology, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Mn2+ alters peroxidase profiles and lignin degradation by the white-rot fungus Pleurotus ostreatus under different nutritional and growth conditions
The white-rot fungus Pleurotus ostreatus produces two types of extracellular peroxidases: manganese-dependent peroxidase (MnP) and versatile peroxidase (VP). The effect of Mn2+ on fungal growth, peroxidase activity profiles, and lignin degradation by P. ostreatus was studied in liquid culture and under solid-state fermentation conditions on perlite, the latter resembling the natural growth conditions of this fungus. The fungus was grown in either a defined asparagine-containing basidiomycete selective medium (BSM) or in a rich peptone medium (PM). Biomass production, as determined by respiration experiments in solid-state fermentation and liquid cultures and fungal growth on Petri dishes, was higher in the PM than in the BSM. Mn2+ affected biomass production only in the PM on Petri dishes. In the nonamended PM, high levels of MnP and VP activity were detected relative to the nonamended BSM. Nevertheless, a higher rate of 14C-lignin mineralization was measured in the Mn2+-amended BSM, as determined during the course of 47 d of fermentation. Mn2+ amendment of the PM increased mineralization rate to that obtained in the Mn2+-amended BSM. The enzyme activity profiles of MnP and VP were studied in the BSM using anion-exchange chromatography. In the nonamended BSM, only minute levels of MnP and VP were detected. On Mn2+ amendment, two MnP isoenzymes (B1 and B2) appeared. Isoenzyme B2 was purified and showed 100% identity with the MnP isoenzyme purified in our previous study from PM-solid-state fermentation (P6). P6 was found to be the dominant isoenzyme in terms of activity level and gene expression compared with the VP isoenzymes. Based on these results, we concluded that Mn2+ plays a key role in lignin degradation under different nutritional and growth conditions, since it is required for the production of MnP in P. ostreatus.
Scientific Publication
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