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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
A novel transgenic marker for migrating limb muscle precursors and for vascular smooth muscle cells
Year:
2001
Source of publication :
Developmental Dynamics
Authors :
כהן, דנה
;
.
פיירמן, אלכסנדר
;
.
רייכנשטיין, משה
;
.
שני, משה
;
.
תדהר, אביטל
;
.
Volume :
220
Co-Authors:
Tidhar, A., Institute of Animal Science, Volcani Center, Bet Dagan, Israel
Reichenstein, M., Institute of Animal Science, Volcani Center, Bet Dagan, Israel
Cohen, D., Institute of Animal Science, Volcani Center, Bet Dagan, Israel
Faerman, A., Institute of Animal Science, Volcani Center, Bet Dagan, Israel
Copeland, N.G., Mouse Cancer Genetics Program, NCI-Frederick Cancer Research and Development Center, Frederick, MD, United States
Gilbert, D.J., Mouse Cancer Genetics Program, NCI-Frederick Cancer Research and Development Center, Frederick, MD, United States
Jenkins, N.A., Mouse Cancer Genetics Program, NCI-Frederick Cancer Research and Development Center, Frederick, MD, United States
Shani, M., Institute of Animal Science, Volcani Center, Bet Dagan, Israel, Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
60
To page:
73
(
Total pages:
14
)
Abstract:
A unique pattern of LacZ expression was found in a transgenic mouse line, likely due to regulatory elements at the site of integration. Two new genes flanking the transgene were identified. At early stages of development, the transgene is transiently expressed in ventro-lateral demomyotomal cells migrating from the somites into the limb buds. At late developmental stages and in the adult, lacZ staining marks vascular smooth muscle cells throughout the vascular bed, with the exception of the major elastic arteries, and in pericytes. No expression was detected in skeletal and smooth muscles. Different patterns of expression in vascular smooth muscles was observed at distinct levels of the vascular tree, in arteries as well as in veins. Vessel injury, resulting in stimulation of smooth muscle cells proliferation and migration, is associated with transgene down-regulation. After the formation of neointima thickening, it is reactivated. This transgenic insertion may therefore be used as a useful marker to identify novel physiological cues or genetic elements involved in the regulation of the vascular smooth muscle phenotype(s). It may also provide an experimental tool for studying vasculature and the involvement of pericytes in regulating microvascular homeostasis. © 2001 Wiley-Liss, Inc.
Note:
Related Files :
animal experiment
animal model
Animals
animal tissue
gene expression
Male
mice
Neointima
Transgenes
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22282
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:50
Scientific Publication
A novel transgenic marker for migrating limb muscle precursors and for vascular smooth muscle cells
220
Tidhar, A., Institute of Animal Science, Volcani Center, Bet Dagan, Israel
Reichenstein, M., Institute of Animal Science, Volcani Center, Bet Dagan, Israel
Cohen, D., Institute of Animal Science, Volcani Center, Bet Dagan, Israel
Faerman, A., Institute of Animal Science, Volcani Center, Bet Dagan, Israel
Copeland, N.G., Mouse Cancer Genetics Program, NCI-Frederick Cancer Research and Development Center, Frederick, MD, United States
Gilbert, D.J., Mouse Cancer Genetics Program, NCI-Frederick Cancer Research and Development Center, Frederick, MD, United States
Jenkins, N.A., Mouse Cancer Genetics Program, NCI-Frederick Cancer Research and Development Center, Frederick, MD, United States
Shani, M., Institute of Animal Science, Volcani Center, Bet Dagan, Israel, Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
A novel transgenic marker for migrating limb muscle precursors and for vascular smooth muscle cells
A unique pattern of LacZ expression was found in a transgenic mouse line, likely due to regulatory elements at the site of integration. Two new genes flanking the transgene were identified. At early stages of development, the transgene is transiently expressed in ventro-lateral demomyotomal cells migrating from the somites into the limb buds. At late developmental stages and in the adult, lacZ staining marks vascular smooth muscle cells throughout the vascular bed, with the exception of the major elastic arteries, and in pericytes. No expression was detected in skeletal and smooth muscles. Different patterns of expression in vascular smooth muscles was observed at distinct levels of the vascular tree, in arteries as well as in veins. Vessel injury, resulting in stimulation of smooth muscle cells proliferation and migration, is associated with transgene down-regulation. After the formation of neointima thickening, it is reactivated. This transgenic insertion may therefore be used as a useful marker to identify novel physiological cues or genetic elements involved in the regulation of the vascular smooth muscle phenotype(s). It may also provide an experimental tool for studying vasculature and the involvement of pericytes in regulating microvascular homeostasis. © 2001 Wiley-Liss, Inc.
Scientific Publication
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