חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Role of nitrogen-metabolism genes expressed during pathogenicity of the alkalinizing Colletotrichum gloeosporioides and their differential expression in acidifying pathogens
Year:
2012
Authors :
מיארה, איתי
;
.
פרוסקי, דב
;
.
שרמן, עמיר
;
.
Volume :
25
Co-Authors:
Miyara, I., Department of Postharvest Science of Fresh Produce, ARO, Bet Dagan, Israel, Hebrew University of Jerusalem, Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, Israel
Shnaiderman, C., Department of Postharvest Science of Fresh Produce, ARO, Bet Dagan, Israel, Hebrew University of Jerusalem, Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, Israel
Meng, X., Fruit Tree Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China
Vargas, W.A., Department of Microbiology and Genetics, CIALE, Universidad de Salamanca, Salamanca, Spain
Diaz-Minguez, J.M., Department of Microbiology and Genetics, CIALE, Universidad de Salamanca, Salamanca, Spain
Sherman, A., Genomic Unit, Plant Sciences Institute, ARO, Bet Dagan, Israel
Thon, M., Department of Microbiology and Genetics, CIALE, Universidad de Salamanca, Salamanca, Spain
Prusky, D., Department of Postharvest Science of Fresh Produce, ARO, Bet Dagan, Israel
Facilitators :
From page:
1251
To page:
1263
(
Total pages:
13
)
Abstract:
Pathogens can actively alter fruit pH around the infection site, signaling modulation of pathogenicity-factor expression, as found for alkalinizing (Colletotrichum and Alternaria spp.) and acidifying (Penicillium, Botrytis, and Sclerotinia spp.) fungi. The nitrogen-metabolism genes GDH2, GS1, GLT, and MEP genes are differentially expressed during colonization by Colletotrichum gloeosporioides, and a Δgdh2 strain reduces ammonia accumulation and pathogenicity. We analyzed the contribution of transporters GLT and MEPB to C. gloeosporiodes pathogenicity. Germinating spores of Δglt strains showed reduced appressorium formation; those of ΔmepB mutants showed rapid ammonia uptake and accumulation inside the hyphae, indicating deregulated uptake. Both mutants reduced pathogenicity, indicating that these transporters function during alkalinizing species pathogenicity. We compared the expressions of these genes in C. gloeosporioides and Sclerotinia sclerotiorum, and found five to 10-fold higher expression at the transcript level in the former. Interestingly, GLT and MEPB in the alkalinizing species showed no and very low sequence identity, respectively, with their counterparts in the acidifying species. Knockout analysis of GLT and MEPB and their differential transcript regulation in the alkalinizing and acidifying species suggest that the ammonia accumulation contributing to pathogenicity in the former is modulated by factors at the gene-regulation levels that are lacking in the acidifying species. © 2012 The American Phytopathological Society.
Note:
Related Files :
Alternaria
Botrytis
Colletotrichum
fungi
Genetics
metabolism
molecular genetics
Penicillium
Sclerotinia sclerotiorum
עוד תגיות
תוכן קשור
More details
DOI :
10.1094/MPMI-01-12-0017-R
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22320
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:50
You may also be interested in
Scientific Publication
Role of nitrogen-metabolism genes expressed during pathogenicity of the alkalinizing Colletotrichum gloeosporioides and their differential expression in acidifying pathogens
25
Miyara, I., Department of Postharvest Science of Fresh Produce, ARO, Bet Dagan, Israel, Hebrew University of Jerusalem, Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, Israel
Shnaiderman, C., Department of Postharvest Science of Fresh Produce, ARO, Bet Dagan, Israel, Hebrew University of Jerusalem, Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, Israel
Meng, X., Fruit Tree Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China
Vargas, W.A., Department of Microbiology and Genetics, CIALE, Universidad de Salamanca, Salamanca, Spain
Diaz-Minguez, J.M., Department of Microbiology and Genetics, CIALE, Universidad de Salamanca, Salamanca, Spain
Sherman, A., Genomic Unit, Plant Sciences Institute, ARO, Bet Dagan, Israel
Thon, M., Department of Microbiology and Genetics, CIALE, Universidad de Salamanca, Salamanca, Spain
Prusky, D., Department of Postharvest Science of Fresh Produce, ARO, Bet Dagan, Israel
Role of nitrogen-metabolism genes expressed during pathogenicity of the alkalinizing Colletotrichum gloeosporioides and their differential expression in acidifying pathogens
Pathogens can actively alter fruit pH around the infection site, signaling modulation of pathogenicity-factor expression, as found for alkalinizing (Colletotrichum and Alternaria spp.) and acidifying (Penicillium, Botrytis, and Sclerotinia spp.) fungi. The nitrogen-metabolism genes GDH2, GS1, GLT, and MEP genes are differentially expressed during colonization by Colletotrichum gloeosporioides, and a Δgdh2 strain reduces ammonia accumulation and pathogenicity. We analyzed the contribution of transporters GLT and MEPB to C. gloeosporiodes pathogenicity. Germinating spores of Δglt strains showed reduced appressorium formation; those of ΔmepB mutants showed rapid ammonia uptake and accumulation inside the hyphae, indicating deregulated uptake. Both mutants reduced pathogenicity, indicating that these transporters function during alkalinizing species pathogenicity. We compared the expressions of these genes in C. gloeosporioides and Sclerotinia sclerotiorum, and found five to 10-fold higher expression at the transcript level in the former. Interestingly, GLT and MEPB in the alkalinizing species showed no and very low sequence identity, respectively, with their counterparts in the acidifying species. Knockout analysis of GLT and MEPB and their differential transcript regulation in the alkalinizing and acidifying species suggest that the ammonia accumulation contributing to pathogenicity in the former is modulated by factors at the gene-regulation levels that are lacking in the acidifying species. © 2012 The American Phytopathological Society.
Scientific Publication
נגישות
menu      
You may also be interested in