נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Allelic variation in genes contributing to glycoalkaloid biosynthesis in a diploid interspecific population of potato
Year:
2014
Source of publication :
Theoretical and Applied Genetics
Authors :
גינזברג, עידית
;
.
Volume :
127
Co-Authors:
Manrique-Carpintero, N.C., Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States
Tokuhisa, J.G., Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States
Ginzberg, I., Department of Vegetable Research, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Veilleux, R.E., Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States
Facilitators :
From page:
391
To page:
405
(
Total pages:
15
)
Abstract:
Genetic factors associated with the biosynthesis and accumulation of steroidal glycoalkaloids (SGAs) in potato were addressed by a candidate gene approach and whole genome single nucleotide polymorphism (SNP) genotyping. Allelic sequences spanning coding regions of four candidate genes [3-hydroxy-3-methylglutaryl coenzyme A reductase 2 (HMG2); 2,3-squalene epoxidase; solanidine galactosyltransferase; and solanidine glucosyltransferase (SGT2)] were obtained from two potato species differing in SGA composition: Solanum chacoense (chc 80-1) and Solanum tuberosum group Phureja (phu DH). An F2 population was genotyped and foliar SGAs quantified. The concentrations of α-solanine, α-chaconine, leptine I, leptine II and total SGAs varied broadly among F2 individuals. F2 plants with chc 80-1 alleles for HMG2 or SGT2 accumulated significantly greater leptines and total SGAs compared to plants with phu DH alleles. Plants with chc 80-1 alleles at both loci expressed the greatest levels of total SGAs, α-solanine and α-chaconine. A significant positive correlation was found between α-solanine and α-chaconine accumulation as well as between leptine I and leptine II. A whole genome SNP genotyping analysis of an F2 subsample verified the importance of chc 80-1 alleles at HMG2 and SGT2 for SGA synthesis and accumulation and suggested additional candidate genes including some previously associated with SGA production. Loci on five and seven potato pseudochromosomes were associated with synthesis and accumulation of SGAs, respectively. Two loci, on pseudochromosomes 1 and 6, explained phenotypic segregation of α-solanine and α-chaconine synthesis. Knowledge of the genetic factors influencing SGA production in potato may assist breeding for pest resistance. © Springer-Verlag Berlin Heidelberg 2013.
Note:
Related Files :
3-hydroxy-3-methylglutaryl coenzyme a reductase
Alleles
Biochemistry
biosynthesis
Genes
Genetics
plant gene
Solanum tuberosum
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00122-013-2226-2
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22359
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:51
You may also be interested in
Scientific Publication
Allelic variation in genes contributing to glycoalkaloid biosynthesis in a diploid interspecific population of potato
127
Manrique-Carpintero, N.C., Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States
Tokuhisa, J.G., Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States
Ginzberg, I., Department of Vegetable Research, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Veilleux, R.E., Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States
Allelic variation in genes contributing to glycoalkaloid biosynthesis in a diploid interspecific population of potato
Genetic factors associated with the biosynthesis and accumulation of steroidal glycoalkaloids (SGAs) in potato were addressed by a candidate gene approach and whole genome single nucleotide polymorphism (SNP) genotyping. Allelic sequences spanning coding regions of four candidate genes [3-hydroxy-3-methylglutaryl coenzyme A reductase 2 (HMG2); 2,3-squalene epoxidase; solanidine galactosyltransferase; and solanidine glucosyltransferase (SGT2)] were obtained from two potato species differing in SGA composition: Solanum chacoense (chc 80-1) and Solanum tuberosum group Phureja (phu DH). An F2 population was genotyped and foliar SGAs quantified. The concentrations of α-solanine, α-chaconine, leptine I, leptine II and total SGAs varied broadly among F2 individuals. F2 plants with chc 80-1 alleles for HMG2 or SGT2 accumulated significantly greater leptines and total SGAs compared to plants with phu DH alleles. Plants with chc 80-1 alleles at both loci expressed the greatest levels of total SGAs, α-solanine and α-chaconine. A significant positive correlation was found between α-solanine and α-chaconine accumulation as well as between leptine I and leptine II. A whole genome SNP genotyping analysis of an F2 subsample verified the importance of chc 80-1 alleles at HMG2 and SGT2 for SGA synthesis and accumulation and suggested additional candidate genes including some previously associated with SGA production. Loci on five and seven potato pseudochromosomes were associated with synthesis and accumulation of SGAs, respectively. Two loci, on pseudochromosomes 1 and 6, explained phenotypic segregation of α-solanine and α-chaconine synthesis. Knowledge of the genetic factors influencing SGA production in potato may assist breeding for pest resistance. © Springer-Verlag Berlin Heidelberg 2013.
Scientific Publication
You may also be interested in