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Kroupitski, Y., Department of Food Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Beth-Dagan 50250, Israel, Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel
Golberg, D., Department of Food Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Beth-Dagan 50250, Israel
Belausov, E., Confocal Microscopy Unit, Agricultural Research Organization, Volcani Center, Beth-Dagan, Israel
Pinto, R., Department of Food Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Beth-Dagan 50250, Israel
Swartzberg, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Beth-Dagan 50250, Israel
Granot, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Beth-Dagan 50250, Israel
Sela, S., Department of Food Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Beth-Dagan 50250, Israel

Outbreaks of salmonellosis related to consumption of fresh produce have raised interest in Salmonella-plant interactions leading to plant colonization. Incubation of gfp-tagged Salmonella enterica with iceberg lettuce leaves in the light resulted in aggregation of bacteria near open stomata and invasion into the inner leaf tissue. In contrast, incubation in the dark resulted in a scattered attachment pattern and very poor stomatal internalization. Forcing stomatal opening in the dark by fusicoccin had no significant effect on Salmonella internalization. These results imply that the pathogen is attracted to nutrients produced de novo by photosynthetically active cells. Indeed, mutations affecting Salmonella motility and chemotaxis significantly inhibited bacterial internalization. These findings suggest a mechanistic account for entry of Salmonella into the plant's apoplast and imply that either Salmonella antigens are not well recognized by the stoma-based innate immunity or that this pathogen has evolved means to evade it. Internalization of leaves may provide a partial explanation for the failure of sanitizers to efficiently eradicate food-borne pathogens in leafy greens. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
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הספר "אוצר וולקני"
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תנאי שימוש
Internalization of Salmonella enterica in leaves is induced by light and involves chemotaxis and penetration through open stomata
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Kroupitski, Y., Department of Food Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Beth-Dagan 50250, Israel, Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel
Golberg, D., Department of Food Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Beth-Dagan 50250, Israel
Belausov, E., Confocal Microscopy Unit, Agricultural Research Organization, Volcani Center, Beth-Dagan, Israel
Pinto, R., Department of Food Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Beth-Dagan 50250, Israel
Swartzberg, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Beth-Dagan 50250, Israel
Granot, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Beth-Dagan 50250, Israel
Sela, S., Department of Food Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Beth-Dagan 50250, Israel

Internalization of Salmonella enterica in leaves is induced by light and involves chemotaxis and penetration through open stomata
Outbreaks of salmonellosis related to consumption of fresh produce have raised interest in Salmonella-plant interactions leading to plant colonization. Incubation of gfp-tagged Salmonella enterica with iceberg lettuce leaves in the light resulted in aggregation of bacteria near open stomata and invasion into the inner leaf tissue. In contrast, incubation in the dark resulted in a scattered attachment pattern and very poor stomatal internalization. Forcing stomatal opening in the dark by fusicoccin had no significant effect on Salmonella internalization. These results imply that the pathogen is attracted to nutrients produced de novo by photosynthetically active cells. Indeed, mutations affecting Salmonella motility and chemotaxis significantly inhibited bacterial internalization. These findings suggest a mechanistic account for entry of Salmonella into the plant's apoplast and imply that either Salmonella antigens are not well recognized by the stoma-based innate immunity or that this pathogen has evolved means to evade it. Internalization of leaves may provide a partial explanation for the failure of sanitizers to efficiently eradicate food-borne pathogens in leafy greens. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Scientific Publication
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