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פותח על ידי קלירמאש פתרונות בע"מ -
Spatial distribution and differential expression of the PBAN receptor in tissues of adult Helicoverpa spp. (Lepidoptera: Noctuidae)
Year:
2007
Source of publication :
Insect Molecular Biology
Authors :
בקר, לירון
;
.
רפאלי, עדה
;
.
Volume :
16
Co-Authors:
Rafaeli, A., Institute for Technology and Storage of Agricultural Products, Agriculture Research Organization, Volcani Center, Bet Dagan, Israel, ARO, Volcani Center, Institute for Technology and Storage of Agricultural Products, PO Box 6, Bet-Dagan 50250, Israel
Bober, R., Institute for Technology and Storage of Agricultural Products, Agriculture Research Organization, Volcani Center, Bet Dagan, Israel
Becker, L., Institute for Technology and Storage of Agricultural Products, Agriculture Research Organization, Volcani Center, Bet Dagan, Israel
Choi, M.-Y., Department of Entomology, Iowa State University, Ames, IA, United States
Fuerst, E.-J., Department of Entomology, Iowa State University, Ames, IA, United States
Jurenka, R., Department of Entomology, Iowa State University, Ames, IA, United States
Facilitators :
From page:
287
To page:
293
(
Total pages:
7
)
Abstract:
Pheromone-biosynthesis-activating neuropeptide (PBAN) regulates sex pheromone production in many female moths. PBAN-like peptides, with common FXPRLamide C-terminals are found in other insect groups where they have other functions. The ubiquity and multifunctional nature of the pyrokinin/PBAN family of peptides suggests that the PBAN receptor proteins could also be present in a variety of insect tissues with alternative functions from that of sex pheromone biosynthesis. Previously we showed the presence of the PBAN-R in Helicoverpa armigera at the protein level. In the present study we confirm the similarities between the two Helicoverpa species: armigera and zea by (1) demonstrating the presence of the receptor protein in Sf9 cells, cloned to express the HezPBAN receptor, as compared with the endogenous receptor protein, previously shown in H. armigera pheromone glands, and (2) by identifying the nucleotide sequence of the PBAN-R from mRNA of H. armigera pheromone glands. Sequences of the two Helicoverpa spp. are 98% identical with most changes taking place in the 3′-end. We demonstrate the spatial distribution of the PBAN receptor protein in membranes of H. armigera brain (Br), thoracic ganglion (TG) and ventral nerve cord (VNC). We also demonstrate the presence and differential expression of the PBAN receptor gene (using reverse transcription-polymerase chain reaction and reverse transcription-quantitative real-time polymerase chain reaction, respectively) in the neural tissues (Br, TG and VNC) of adult H. armigera female moths as compared with its presence in pheromone glands. Surprisingly, the gene for the PBAN receptor is also detected in the male tissue homologous to the female pheromone gland, the aedeagus, although the protein is undetectable and PBAN does not induce physiological (pheromone production) or cellular (cyclic-adenosine monophosphate production) responses in this tissue. Our findings indicate that PBAN or PBAN-like receptors are present in the neural tissues and may represent a neurotransmitter-like function for PBAN-like peptides. In addition, the surprising discovery of the presence of the gene encoding the PBAN receptor in the male homologous tissue, but its absence at the protein level, launches opportunities for studying molecular regulation pathways and the evolution of these G protein coupled receptors (GPCRs). © 2007 The Authors.
Note:
Related Files :
Animal
Animals
Female
Genetics
Lepidoptera
Male
metabolism
molecular genetics
neuropeptide
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1365-2583.2007.00725.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22432
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:51
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Scientific Publication
Spatial distribution and differential expression of the PBAN receptor in tissues of adult Helicoverpa spp. (Lepidoptera: Noctuidae)
16
Rafaeli, A., Institute for Technology and Storage of Agricultural Products, Agriculture Research Organization, Volcani Center, Bet Dagan, Israel, ARO, Volcani Center, Institute for Technology and Storage of Agricultural Products, PO Box 6, Bet-Dagan 50250, Israel
Bober, R., Institute for Technology and Storage of Agricultural Products, Agriculture Research Organization, Volcani Center, Bet Dagan, Israel
Becker, L., Institute for Technology and Storage of Agricultural Products, Agriculture Research Organization, Volcani Center, Bet Dagan, Israel
Choi, M.-Y., Department of Entomology, Iowa State University, Ames, IA, United States
Fuerst, E.-J., Department of Entomology, Iowa State University, Ames, IA, United States
Jurenka, R., Department of Entomology, Iowa State University, Ames, IA, United States
Spatial distribution and differential expression of the PBAN receptor in tissues of adult Helicoverpa spp. (Lepidoptera: Noctuidae)
Pheromone-biosynthesis-activating neuropeptide (PBAN) regulates sex pheromone production in many female moths. PBAN-like peptides, with common FXPRLamide C-terminals are found in other insect groups where they have other functions. The ubiquity and multifunctional nature of the pyrokinin/PBAN family of peptides suggests that the PBAN receptor proteins could also be present in a variety of insect tissues with alternative functions from that of sex pheromone biosynthesis. Previously we showed the presence of the PBAN-R in Helicoverpa armigera at the protein level. In the present study we confirm the similarities between the two Helicoverpa species: armigera and zea by (1) demonstrating the presence of the receptor protein in Sf9 cells, cloned to express the HezPBAN receptor, as compared with the endogenous receptor protein, previously shown in H. armigera pheromone glands, and (2) by identifying the nucleotide sequence of the PBAN-R from mRNA of H. armigera pheromone glands. Sequences of the two Helicoverpa spp. are 98% identical with most changes taking place in the 3′-end. We demonstrate the spatial distribution of the PBAN receptor protein in membranes of H. armigera brain (Br), thoracic ganglion (TG) and ventral nerve cord (VNC). We also demonstrate the presence and differential expression of the PBAN receptor gene (using reverse transcription-polymerase chain reaction and reverse transcription-quantitative real-time polymerase chain reaction, respectively) in the neural tissues (Br, TG and VNC) of adult H. armigera female moths as compared with its presence in pheromone glands. Surprisingly, the gene for the PBAN receptor is also detected in the male tissue homologous to the female pheromone gland, the aedeagus, although the protein is undetectable and PBAN does not induce physiological (pheromone production) or cellular (cyclic-adenosine monophosphate production) responses in this tissue. Our findings indicate that PBAN or PBAN-like receptors are present in the neural tissues and may represent a neurotransmitter-like function for PBAN-like peptides. In addition, the surprising discovery of the presence of the gene encoding the PBAN receptor in the male homologous tissue, but its absence at the protein level, launches opportunities for studying molecular regulation pathways and the evolution of these G protein coupled receptors (GPCRs). © 2007 The Authors.
Scientific Publication
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