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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
The conserved FRNK box in HC-Pro, a plant viral suppressor of gene silencing, is required for small RNA binding and mediates symptom development
Year:
2007
Source of publication :
Journal of Virology
Authors :
ארזי, צחי
;
.
גאבה, ויקטור
;
.
גל-און, עמית
;
.
חרונסקי, אלינה
;
.
ליבמן, דיאנה
;
.
Volume :
81
Co-Authors:
Shiboleth, Y.M., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Haronsky, E., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Leibman, D., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Arazi, T., Department of Ornamental Horticulture, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Wassenegger, M., AgroScience GmbH, AlPlanta-Institute for Plant Research, Breitenweg 71, 67435 Neustadt, Germany
Whitham, S.A., Department of Plant Pathology, Iowa State University, Ames, IA 50011-1020, United States
Gaba, V., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Gal-On, A., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel, Department of Plant Pathology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
13135
To page:
13148
(
Total pages:
14
)
Abstract:
The helper component-proteinase (HC-Pro) protein of potyviruses is a suppressor of gene silencing and has been shown to elicit plant developmental-defect-like symptoms. In Zucchini yellow mosaic virus (ZYMV), a mutation in the highly conserved FR180NK box of HC-Pro to FI 180NK causes attenuation of these symptoms. At 5 days postinoculation and before symptoms appear, virus accumulation, HC-Pro protein levels, and viral short interfering RNA (siRNA) levels are similar for the severe (FRNK) and attenuated (FINK) strains. At this stage, ZYMVFRNK caused greater accumulation of most microRNAs (miRNAs), and especially of their complementary miRNA "passenger" strands (miRNA*s), in systemically infected leaves than the attenuated ZYMVFINK did. HC-ProFRNK specifically bound artificial siRNA and miRNA/miRNA* duplexes with a much higher affinity than the mutated HC-ProFINK. Further analysis of the mutant and wild-type HC-Pro proteins revealed that suppressor activity of the ZYMV HCFINK mutant was not diminished. However, the FINK mutation caused a loss of HC-Pro suppressor function in other potyviruses. Replacement of the second positively charged amino acid in the ZYMV FRNK box to result in FRNA also caused symptom attenuation and reduced small RNA duplex-binding affinity without loss of suppressor activity. Our data suggest that the highly conserved FRNK box in the HC-Pro of potyviruses is a probable point of contact with siRNA and miRNA duplexes. The interaction of the FRNK box with populations of miRNAs directly influences their accumulation levels and regulatory functions, resulting in symptom development. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Note:
Related Files :
Cucurbita
Cysteine Endopeptidases
gene silencing
mutation
protein HC Pro
symptom
Zucchini yellow mosaic virus
עוד תגיות
תוכן קשור
More details
DOI :
10.1128/JVI.01031-07
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22439
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:51
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Scientific Publication
The conserved FRNK box in HC-Pro, a plant viral suppressor of gene silencing, is required for small RNA binding and mediates symptom development
81
Shiboleth, Y.M., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Haronsky, E., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Leibman, D., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Arazi, T., Department of Ornamental Horticulture, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Wassenegger, M., AgroScience GmbH, AlPlanta-Institute for Plant Research, Breitenweg 71, 67435 Neustadt, Germany
Whitham, S.A., Department of Plant Pathology, Iowa State University, Ames, IA 50011-1020, United States
Gaba, V., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Gal-On, A., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel, Department of Plant Pathology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
The conserved FRNK box in HC-Pro, a plant viral suppressor of gene silencing, is required for small RNA binding and mediates symptom development
The helper component-proteinase (HC-Pro) protein of potyviruses is a suppressor of gene silencing and has been shown to elicit plant developmental-defect-like symptoms. In Zucchini yellow mosaic virus (ZYMV), a mutation in the highly conserved FR180NK box of HC-Pro to FI 180NK causes attenuation of these symptoms. At 5 days postinoculation and before symptoms appear, virus accumulation, HC-Pro protein levels, and viral short interfering RNA (siRNA) levels are similar for the severe (FRNK) and attenuated (FINK) strains. At this stage, ZYMVFRNK caused greater accumulation of most microRNAs (miRNAs), and especially of their complementary miRNA "passenger" strands (miRNA*s), in systemically infected leaves than the attenuated ZYMVFINK did. HC-ProFRNK specifically bound artificial siRNA and miRNA/miRNA* duplexes with a much higher affinity than the mutated HC-ProFINK. Further analysis of the mutant and wild-type HC-Pro proteins revealed that suppressor activity of the ZYMV HCFINK mutant was not diminished. However, the FINK mutation caused a loss of HC-Pro suppressor function in other potyviruses. Replacement of the second positively charged amino acid in the ZYMV FRNK box to result in FRNA also caused symptom attenuation and reduced small RNA duplex-binding affinity without loss of suppressor activity. Our data suggest that the highly conserved FRNK box in the HC-Pro of potyviruses is a probable point of contact with siRNA and miRNA duplexes. The interaction of the FRNK box with populations of miRNAs directly influences their accumulation levels and regulatory functions, resulting in symptom development. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Scientific Publication
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