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Journal of Virology
Shiboleth, Y.M., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Haronsky, E., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Leibman, D., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Arazi, T., Department of Ornamental Horticulture, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Wassenegger, M., AgroScience GmbH, AlPlanta-Institute for Plant Research, Breitenweg 71, 67435 Neustadt, Germany
Whitham, S.A., Department of Plant Pathology, Iowa State University, Ames, IA 50011-1020, United States
Gaba, V., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Gal-On, A., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel, Department of Plant Pathology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
The helper component-proteinase (HC-Pro) protein of potyviruses is a suppressor of gene silencing and has been shown to elicit plant developmental-defect-like symptoms. In Zucchini yellow mosaic virus (ZYMV), a mutation in the highly conserved FR180NK box of HC-Pro to FI 180NK causes attenuation of these symptoms. At 5 days postinoculation and before symptoms appear, virus accumulation, HC-Pro protein levels, and viral short interfering RNA (siRNA) levels are similar for the severe (FRNK) and attenuated (FINK) strains. At this stage, ZYMVFRNK caused greater accumulation of most microRNAs (miRNAs), and especially of their complementary miRNA "passenger" strands (miRNA*s), in systemically infected leaves than the attenuated ZYMVFINK did. HC-ProFRNK specifically bound artificial siRNA and miRNA/miRNA* duplexes with a much higher affinity than the mutated HC-ProFINK. Further analysis of the mutant and wild-type HC-Pro proteins revealed that suppressor activity of the ZYMV HCFINK mutant was not diminished. However, the FINK mutation caused a loss of HC-Pro suppressor function in other potyviruses. Replacement of the second positively charged amino acid in the ZYMV FRNK box to result in FRNA also caused symptom attenuation and reduced small RNA duplex-binding affinity without loss of suppressor activity. Our data suggest that the highly conserved FRNK box in the HC-Pro of potyviruses is a probable point of contact with siRNA and miRNA duplexes. The interaction of the FRNK box with populations of miRNAs directly influences their accumulation levels and regulatory functions, resulting in symptom development. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
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הספר "אוצר וולקני"
אודות
תנאי שימוש
The conserved FRNK box in HC-Pro, a plant viral suppressor of gene silencing, is required for small RNA binding and mediates symptom development
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Shiboleth, Y.M., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Haronsky, E., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Leibman, D., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Arazi, T., Department of Ornamental Horticulture, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Wassenegger, M., AgroScience GmbH, AlPlanta-Institute for Plant Research, Breitenweg 71, 67435 Neustadt, Germany
Whitham, S.A., Department of Plant Pathology, Iowa State University, Ames, IA 50011-1020, United States
Gaba, V., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Gal-On, A., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel, Department of Plant Pathology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
The conserved FRNK box in HC-Pro, a plant viral suppressor of gene silencing, is required for small RNA binding and mediates symptom development
The helper component-proteinase (HC-Pro) protein of potyviruses is a suppressor of gene silencing and has been shown to elicit plant developmental-defect-like symptoms. In Zucchini yellow mosaic virus (ZYMV), a mutation in the highly conserved FR180NK box of HC-Pro to FI 180NK causes attenuation of these symptoms. At 5 days postinoculation and before symptoms appear, virus accumulation, HC-Pro protein levels, and viral short interfering RNA (siRNA) levels are similar for the severe (FRNK) and attenuated (FINK) strains. At this stage, ZYMVFRNK caused greater accumulation of most microRNAs (miRNAs), and especially of their complementary miRNA "passenger" strands (miRNA*s), in systemically infected leaves than the attenuated ZYMVFINK did. HC-ProFRNK specifically bound artificial siRNA and miRNA/miRNA* duplexes with a much higher affinity than the mutated HC-ProFINK. Further analysis of the mutant and wild-type HC-Pro proteins revealed that suppressor activity of the ZYMV HCFINK mutant was not diminished. However, the FINK mutation caused a loss of HC-Pro suppressor function in other potyviruses. Replacement of the second positively charged amino acid in the ZYMV FRNK box to result in FRNA also caused symptom attenuation and reduced small RNA duplex-binding affinity without loss of suppressor activity. Our data suggest that the highly conserved FRNK box in the HC-Pro of potyviruses is a probable point of contact with siRNA and miRNA duplexes. The interaction of the FRNK box with populations of miRNAs directly influences their accumulation levels and regulatory functions, resulting in symptom development. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
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