אסיף מאגר המחקר החקלאי

Transgenic tomato plants expressing the tomato bellow leaf curl virus capsid protein are resistant to the virus (from: Bio/Technology)

Year:

1994

Source of publication :

Authors :

Volume :

12

Co-Authors:

Zamir, D., The Hebrew University of Jerusalem, Faculty of Agriculture, Department of Field and Vegetable Crops and the Otto Warburg Center for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76100, Israel
Navot, N., The Hebrew University of Jerusalem, Faculty of Agriculture, Department of Field and Vegetable Crops and the Otto Warburg Center for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76100, Israel
Zeidan, M., The Hebrew University of Jerusalem, Faculty of Agriculture, Department of Field and Vegetable Crops and the Otto Warburg Center for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76100, Israel
Michelson, I., The Hebrew University of Jerusalem, Faculty of Agriculture, Department of Field and Vegetable Crops and the Otto Warburg Center for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76100, Israe
Gafni, Y., Department of Genetics, Agricultural Research Organization, P.O. Box 6, Bet Dagan, 50250, Israel
Czosnek, H., The Hebrew University of Jerusalem, Faculty of Agriculture, Department of Field and Vegetable Crops and the Otto Warburg Center for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76100, Israel

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Abstract:

The tomato yellow leaf curl virus (TYLCV) gene that encodes the capsid protein (VI) was placed under transcriptional control of the cauliflower mosaic virus 35S promoter and cloned into an Agrobacterium Ti-derived plasmid and used to transform plants from an interspecific tomato hybrid, Lycopersicon esculentum X L. pennella (Fl), sensitive to the TYLCV disease. When transgenic Fl plants, expressing the VI gene, were inoculated with TYLCV using whiteflies fed on TYLCV-infected plants, they responded either as untransformed tomato or showed expression of delayed disease symptoms and recovery from the disease with increasingly more resistance upon repeated inoculation. Transformed plants that were as sensitive to inoculation as untransformed controls expressed the VI gene at the RNA level only. All the transformed plants that recovered from disease expressed the TYLCV capsid protein. © 1994 Nature Publishing Group. All rights reserved.

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